Catalog No: OKEH07557
Size:96T
Price: $725.00
SKU
OKEH07557
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology KLF2 ELISA Kit (Human) (OKEH07557) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLF2 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 0.78-50ng/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=4.9% (n = 20) Mean Inter-assay CV%: <=5.6% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription. | ||||||||||||||||||||||
:: | Subcellular Location: Nucleus | ||||||||||||||||||||||
:: | Unigene: Hs.685136 SMR: Q9Y5W3 String: 9606.ENSP00000248071 | ||||||||||||||||||||||
:: | KEGG: hsa:10365 MIM: 602016 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 0.35ng/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Krueppel-like factor 2 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | KLF2 |
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Gene Full Name | Kruppel like factor 2 |
Alias Symbols | Krueppel-like factor 2;Kruppel-like factor 2 (lung);Kruppel-like factor LKLF;LKLF;lung krueppel-like factor;lung Kruppel-like factor;lung Kruppel-like zinc finger transcription factor. |
NCBI Gene Id | 10365 |
Protein Name | Q9Y5W3 |
Description of Target | Krueppel-like factor 2 |
Uniprot ID | https://www.uniprot.org/uniprot/Q9Y5W3 |
Protein Accession # | NP_057354.1 |
Nucleotide Accession # | NM_016270.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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