KLF2 Antibody (OAAB19506)

Catalog No: OAAB19506

Size:100ul

Western blot analysis of lysate from human heart tissue lysate, using KLF2 Antibody (OAAB19506). OAAB19506 was diluted at 1: 500. A goat anti-rabbit IgG H&L (HRP) at 1: 5000 dilution was used as the secondary antibody.Lysate at 35ug.

Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min; Incubation: Anti-KLF2 Polyclonal Antibody, Unconjugated1: 200, overnight at 4C, followed by conjugation to the secondary antibody and DAB staining

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Rabbit, Sheep
• Product Format: Liquid 0.01M TBS(pH7.4) with 0.09% sodium azide and 50% Glycerol
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB, IHC
  Cellular Location: Nucleus
• Reconstitution and Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody. The antibody is stable for at least two weeks at 2-4°C.
• Concentration: 1 mg/ml
• Epitope: 251-355aa
• Application Info: WB: 1:500
  IHC: 1:100~500
• Reference: Kozyrev S.V.,et al.FEBS Lett. 448:149-152(1999).
  Wani M.A.,et al.Genomics 60:78-86(1999).
  Lee H.J.,et al.Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
  Conkright M.D.,et al.J. Biol. Chem. 276:29299-29306(2001).
  

Target Info

• Gene Symbol: KLF2
• Alias Symbols: LKLF
• NCBI Gene Id: 10365
• Description of Target: Binds to the CACCC box in the beta-globin gene promoter and activates transcription.
• Uniprot ID: Q9Y5W3
• Molecular Weight: 37 kDa