KLF14 ELISA Kit (Human) (OKEH04167)

Catalog No: OKEH04167

Size:96T

Human KLF14 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology KLF14 ELISA Kit (Human) (OKEH04167) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KLF14 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KLF14 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KLF14 captured in the well.
• ELISA Kit Range: 0.156-10nmol/L
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 4.3% (n = 20)
  Mean Inter-assay CV%: <= 9.8% (n = 20)
• ELISA Kit Component:
  • KLF14 Microplate: 96 Wells (12 x 8 Well Strips)
  • KLF14 Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • KLF14 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.085nmol/L
• Specificity: Natural and recombinant Human Krueppel-like factor 14
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KLF14
• Gene Full Name: Kruppel like factor 14
• Alias Symbols: basic transcription element-binding protein 5;BTEB5;BTE-binding protein 5;Krueppel-like factor 14;transcription factor BTEB5.
• NCBI Gene Id: 136259
• Protein Name: Q8TD94
• Description of Target: Krueppel-like factor 14
• Uniprot ID: https://www.uniprot.org/uniprot/Q8TD94
• Protein Accession #: NP_619638.2
• Nucleotide Accession #: NM_138693.3
• Protein Size (# AA): 323
• Molecular Weight: 33 kDa