Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD06671
Size:96WELLS
Price: $600.00
SKU
OKCD06671
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for KLF10 ELISA Kit (Human) (OKCD06671)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF Beta Inducible Early Response Gene 1 (TIEG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGF Beta Inducible Early Response Gene 1 (TIEG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.312-20ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGF Beta Inducible Early Response Gene 1 (TIEG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGF Beta Inducible Early Response Gene 1 (TIEG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
KLF10 Microplate96 Wells (12 x 8 Well strips)
KLF10 Lyophilized Standard2
100X KLF10 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids.
Sensitivity< 0.124ng/mL
Gene SymbolKLF10
Gene Full NameKruppel like factor 10
Alias Symbolsearly growth response-alpha;EGRA;EGR-alpha;Krueppel-like factor 10;TGFB-inducible early growth response protein 1;TIEG;TIEG1;transforming growth factor-beta-inducible early growth response protein 1;zinc finger transcription factor TIEG.
NCBI Gene Id7071
Protein NameKrueppel-like factor 10
Description of TargetTranscriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.
Uniprot IDQ13118
Protein Accession #NP_001027453.1
Nucleotide Accession #NM_001032282.3
Write Your Own Review
You're reviewing:KLF10 ELISA Kit (Human) (OKCD06671)
Your Rating
We found other products you might like!