Catalog No: OKCD06671
Size:96WELLS
Price: $600.00
SKU
OKCD06671
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
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Datasheets/Manuals | Printable datasheet for KLF10 ELISA Kit (Human) (OKCD06671) |
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Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to TGF Beta Inducible Early Response Gene 1 (TIEG1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain TGF Beta Inducible Early Response Gene 1 (TIEG1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of TGF Beta Inducible Early Response Gene 1 (TIEG1) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 0.312-20ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level TGF Beta Inducible Early Response Gene 1 (TIEG1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level TGF Beta Inducible Early Response Gene 1 (TIEG1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
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Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.124ng/mL |
Gene Symbol | KLF10 |
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Gene Full Name | Kruppel like factor 10 |
Alias Symbols | early growth response-alpha;EGRA;EGR-alpha;Krueppel-like factor 10;TGFB-inducible early growth response protein 1;TIEG;TIEG1;transforming growth factor-beta-inducible early growth response protein 1;zinc finger transcription factor TIEG. |
NCBI Gene Id | 7071 |
Protein Name | Krueppel-like factor 10 |
Description of Target | Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation. |
Uniprot ID | Q13118 |
Protein Accession # | NP_001027453.1 |
Nucleotide Accession # | NM_001032282.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
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