KLF10 Antibody (OAGA03268)

Catalog No: OAGA03268

Size:100 ul

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 ug 293T
B. 30 ug of eGFP-human KLF10-transfected 293T cells
7.5% SDS-PAGE
KLF10 antibody dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

KLF10 antibody detects KLF10 protein by western blot analysis.
A. 30 ug HeLa whole cell lysate/extract
B. 30 ug HeLa nuclear lysate/extract
10% SDS-PAGE
KLF10 antibody dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

KLF10 antibody immunoprecipitates KLF10 protein in IP experiments.
IP Sample: HeLa whole cell lysate/extract
A. Control with 2 ug of preimmune rabbit IgG
B. Immunoprecipitation of KLF10 protein by 2 ug of KLF10 antibody 7.5% SDS-PAGE
The immunoprecipitated KLF10 protein was detected by KLF10 antibody diluted at 1:1000.
EasyBlot anti-rabbit IgG was used as a secondary reagent.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: IP, WB
• Reconstitution and Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human KLF10/TIEG1. The exact sequence is proprietary.
• Purification: Purified by antigen-affinity chromatography.
• Concentration: 1 mg/ml
• Preservative: 0.01% Thimerosal
• Application Info: Suggested starting dilutions are as follows: IP: 1:100-1:500, WB: 1:500-1:10000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
• Storage Buffer: 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Target Info

• Gene Symbol: KLF10
• Gene Full Name: Kruppel like factor 10
• Alias Symbols: EGRA, TIEG, TIEG1, EGR-alpha
• NCBI Gene Id: 7071
• Protein Name: Krueppel-like factor 10
• Description of Target: This gene encodes a member of a family of proteins that feature C2H2-type zinc finger domains. The encoded protein is a transcriptional repressor that acts as an effector of transforming growth factor beta signaling. Activity of this protein may inhibit the growth of cancers, particularly pancreatic cancer. Alternative splicing results in multiple transcript variants.
• Uniprot ID: Q13118
• Protein Accession #: NP_001027453.1
• Nucleotide Accession #: NM_001032282.3