KLF1 Antibody - C-terminal region (OAAB10807)

Catalog No: OAAB10807

Size:400ul

KLF1 Antibody (C-term) (Cat. #OAAB10807) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the KLF1 antibody detected the KLF1 protein (arrow).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB
  Function: Transcription regulator of erythrocyte development that probably serves as a general switch factor during erythropoiesis Is a dual regulator of fetal-to-adult globin switching. Binds to the CACCC box in the beta-globin gene promoter and acts as a preferential activator of this gene. Furthermore, it binds to the BCL11A promoter and activates expression of BCL11A, which in turn represses the HBG1 and HBG2 genes. This dual activity ensures that, in most adults, fetal hemoglobin levels are low. Able to activate CD44 and AQP1 promoters. When sumoylated, acts as a transcriptional repressor by promoting interaction with CDH2/MI2beta and also represses megakaryocytic differentiation.
  Cellular Location: Nucleus. Note=Colocalizes with SUMO1 in nuclear speckles (By similarity)
  Tissue Location: Expression restricted to adult bone marrow and fetal liver. Not expressed in myeloid nor lymphoid cell lines
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This KLF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 248-276 amino acids from the C-terminal region of human KLF1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: For research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000

Target Info

• Gene Symbol: KLF1
• Alias Symbols: EKLF, EKLF/KLF1
• NCBI Gene Id: 10661
• Protein Name: Krueppel-like factor 1
• Description of Target: This gene encodes a hematopoietic-specific transcription factor that induces high-level expression of adult beta-globin and other erythroid genes. The zinc-finger protein binds to the DNA sequence CCACACCCT found in the beta hemoglobin promoter. Heterozygous loss-of-function mutations in this gene result in the dominant In(Lu) blood phenotype.
• Uniprot ID: Q13351
• Protein Accession #: NP_006554.1
• Molecular Weight: 38221 Da