Catalog No: OKCD09095
Size:96WELLS
Price: $825.00
SKU
OKCD09095
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Datasheets/ManualsPrintable datasheet for KL ELISA Kit (Mouse) (OKCD09095)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Klotho (KL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Klotho (KL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range3.12-200pg/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
KL Microplate96 Wells (12 x 8 Well strips)
KL Lyophilized Standard2
100X KL HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates and other biological fluids
Sensitivity< 1.25pg/mL
Gene SymbolKl
Gene Full Nameklotho
Alias Symbolsalp;alpha-kl;klotho;secreted form of Klotho protein.
NCBI Gene Id16591
Protein NameKlotho
Description of TargetMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.
Uniprot IDO35082
Protein Accession #NP_038851.2
Nucleotide Accession #NM_013823.2
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