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Catalog No: OKCD03704
Size:96WELLS
Price: $850.00
SKU
OKCD03704
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KL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03704)

Datasheets/ManualsPrintable datasheet for KL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03704)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)99-105%99-105%80-94%94-105%
EDTA Plasma (n=5)80-102%85-93%83-91%98-105%
Heparin Plasma (n=5)80-93%87-96%80-105%95-103%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Klotho (KL). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Klotho (KL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Klotho (KL) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)90-9893
EDTA Plasma (n=5)84-9892
Heparin Plasma (n=5)99-105102
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Klotho (KL) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Klotho (KL) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Kl Microplate96 Wells (12 x 8 Well strips)
Kl Lyophilized Standard2
100X Kl Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity2.74 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKl
Gene Full Nameklotho
Alias Symbolsalp;alpha-kl;klotho;secreted form of Klotho protein.
NCBI Gene Id16591
Protein NameKlotho
Description of TargetMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 241 and 874, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D. Essential factor for the specific interaction between FGF23 and FGFR1.
Uniprot IDO35082
Protein Accession #NP_038851
Nucleotide Accession #NM_013823
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