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Catalog No: OKCD03913
Size:96WELLS
Price: $850.00
SKU
OKCD03913
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KITL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03913)

Datasheets/ManualsPrintable datasheet for KITL Chemi-Luminescent ELISA Kit (Mouse) (OKCD03913)
Product Info
Predicted Species ReactivityMouse|Mus musculus
ApplicationChemiluminescent immunoassay
ELISA Kit Detection MethodChemiluminescent
ELISA Kit Duration2h, 40min
ELISA Kit Linearity
Sample1:21:41:81:16
Serum (n=5)97-105%81-93%86-94%98-105%
EDTA Plasma (n=5)78-97%99-105%80-103%80-96%
Heparin Plasma (n=5)87-98%93-103%84-91%92-101%
ELISA Kit PrincipleThe microplate provided in this kit has been pre-coated with an antibody specific to Stem Cell Factor (SCF). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Stem Cell Factor (SCF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Stem Cell Factor (SCF) level in the sample or standard.
ELISA Kit Range2.74-2,000pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)79-9185
EDTA Plasma (n=5)88-10391
Heparin Plasma (n=5)92-10597
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stem Cell Factor (SCF) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Kitl Microplate96 Wells (12 x 8 Well strips)
Kitl Lyophilized Standard2
100X Kitl Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity2.74 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKitl
Gene Full Namekit ligand
Alias Symbolsbl;blz;C-kit ligand;Clo;cloud gray;Con;contrasted;Gb;grizzle-belly;hematopoietic growth factor KL;Kit;kit ligand;Kitlg;mast cell growth factor;Mgf;S;SC;SCF;SF;Sl;SLF;Ste;Steel factor;stem cell factor.
NCBI Gene Id17311
Protein NameKit ligand
Description of TargetLigand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Uniprot IDP20826
Protein Accession #NP_001334085
Nucleotide Accession #NM_001347156
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