KIT Antibody : APC-Cy7 (OATA00134)

Catalog No: OATA00134

C57Bl/6 bone marrow cells were stained with violetFluor 450 Anti-Mouse CD45R (B220) (OATA00610) and 0.25 ug APC-Cyanine7 Anti-Mouse CD117 (OATA00134) (right panel) or 0.25 ug APC-Cyanine7 Rat IgG2b (left panel).

Product Info

• Tested Species Reactivity: Mouse
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: ACK2
• Isotype: Rat IgG2b, kappa
• Host: Rat
• Conjugation: APC: Allophycocyanin, CY7: Cyanine 7
• Application: FC
  Excitation Laser: Red (633-640 nm)
  Emmision: 785 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD117 (c-Kit)
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Tang X, Tian L, Esteso G, Choi S-C, Barrow AD, Colonna M, Borrego F, and Coligan JE. 2012. J. Immunol. 188: 548-558. (Flow cytometry)

  Launay J-M, Herve P, Callebert J, Mallat Z, Collet C, Doly S, Belmer A, Diaz SL, Cote F, Humbert M, and Maroteaux L. 2012. Blood. 119: 1772-1780. (Immunohistochemistry - formaldehyde fixed tissue)

  Mark-Kappeler CJ, Sen N, Lukefahr A, McKee L, Sipes IG, Konhilas J, and Hoyer PB. 2011. Biol. Reprod. 85: 755-762. (in vitro blocking, Western blot - Fischer 344 Rat)

  Kim M-H, Granick JL, Wkok C, Walker NJ, Borjesson DL, Curry F-RE, Miller LS, and Simon SI. 2011. Blood. 117:3343-3352. (in vivo depletion)

  Fiorina P, Jurewicz M, Vergani A, Petrelli A, Carvello M, D"™Addio F et al. 2011. J. Immunol. 186:121-131. (in vivo blocking)

  Stanich JE, Gibbons SJ, Eisenman ST, Bardsley MR, Rock JR, Harfe BD, Ordog T, and Farrugia G. 2011. 301: G1044-G1051. (Immunocytochemistry - acetone fixed cells)

  Carlsson IB, Laitinen MPE, Scott JE, Louhio H, Velentzis L, Tuuri T, Aaltonen J, Ritvos O, Winston RML, and Hovatta O. 2006. Reproduction. 131: 641-649. (Immunohistochemistry - paraffin embedded tissue, in vivo blocking)

Target Info

• Gene Symbol: KIT
• Gene Full Name: KIT proto-oncogene receptor tyrosine kinase
• Alias Symbols: W, Bs, SC, SO, Fdc, Ssm, Gsfs, SCO1, SCO5, SOW3, Tr-k, c-KI, CD117, belly, c-KIT, Tr-kit, Gsfsco1, Gsfsco5, Gsfsow3
• NCBI Gene Id: 16590
• Protein Name: mast/stem cell growth factor receptor Kit
• Description of Target: The c-Kit proto-oncogene is the cellular homolog of the transforming gene of a feline retrovirus (v-Kit). The c-kit protein includes characteristics of a protein kinase transmembrane receptor. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P05532
• Protein Accession #: NP_001116205.1
• Nucleotide Accession #: NM_001122733.1