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Catalog No: OKEH07022
Size:96T
Price: $525.00
SKU
OKEH07022
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat, Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD 450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Kiss1r ELISA Kit (Rat) (OKEH07022) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kiss1r has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Kiss1r is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kiss1r captured in the well.
ELISA Kit Range3.12-200ng/mL
ELISA Kit RecoveryMean recovery when spiking into matrix = 95%
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated with 20 replicates of 3 samples representing low, middle and high level LIPC. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level LIPC using 8 replicates on each of 3 plates.
Intra-Assay CV <5.2%
Inter-Assay CV <8.1%
ELISA Kit Component
ComponentAmount
Kiss1r Microplate96 Wells (12 x 8 Well strips)
Kiss1r Lyophilized Standard2 x 200 ng
100X Kiss1r HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity1.67 ng/mL
SpecificityNatural and recombinant Rat KiSS-1 receptor
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKiss1r
Gene Full NameKISS1 receptor
Alias SymbolsG protein-coupled receptor 54;Gpr54;G-protein coupled receptor 54;G-protein coupled receptor OT7T175;kiSS-;kiSS-1 receptor;kiSS-1R;kisspeptins receptor;metastin receptor;orphan G protein-coupled receptor GPR54;rOT7T175.
NCBI Gene Id78976
Protein NameQ924U1
Description of TargetKiSS-1 receptor
Uniprot IDhttps://www.uniprot.org/uniprot/Q924U1
Protein Accession #NP_076482
Nucleotide Accession #NM_023992
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