Catalog No: OKCD02593
Size:96WELLS
Price: $725.00
SKU
OKCD02593
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Datasheets/ManualsPrintable datasheet for KISS1R ELISA Kit (Rat) (OKCD02593)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kisspeptin Receptor (KISS1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kisspeptin Receptor (KISS1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kisspeptin Receptor (KISS1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kisspeptin Receptor (KISS1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kisspeptin Receptor (KISS1R) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kisspeptin Receptor (KISS1R) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KISS1R Microplate96 Wells (12 x 8 Well strips)
KISS1R Lyophilized Standard2
100X Biotinylated KISS1R Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
Stop Solution1 x 6 mL
TMB Substrate1 x 9 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity0.059 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Kisspeptin Receptor (KISS1R). No significant cross-reactivity or interference between Kisspeptin Receptor (KISS1R) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolKiss1r
Gene Full NameKISS1 receptor
Alias SymbolsG protein-coupled receptor 54;Gpr54;G-protein coupled receptor 54;G-protein coupled receptor OT7T175;kiSS-;kiSS-1 receptor;kiSS-1R;kisspeptins receptor;metastin receptor;orphan G protein-coupled receptor GPR54;rOT7T175.
NCBI Gene Id78976
Protein NameKiSS-1 receptor
Description of TargetReceptor for metastin, a C-terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins.
Uniprot IDQ924U1
Protein Accession #NP_076482.2
Nucleotide Accession #NM_023992.2
Protein Size (# AA)396
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