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KISS1 ELISA Kit (Human) : 96 Wells (OKEH01461)

96 Wells
$529.00
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Application:
ELISA
Gene Symbol:
KISS1
Official Gene Full Name:
KiSS-1 metastasis-suppressor
NCBI Gene Id:
3814
Alias Symbols:
KiSS-1, Kisspeptin-1, Metastasis-suppressor KiSS-1, METASTIN, MGC39258, PP5098
Description of Target:
This gene is a metastasis suppressor gene that suppresses metastases of melanomas and breast carcinomas without affecting tumorigenicity. The encoded protein may inhibit chemotaxis and invasion and thereby attenuate metastasis in malignant melanomas. Studies suggest a putative role in the regulation of events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. A protein product of this gene, kisspeptin, stimulates gonadotropin-releasing hormone (GnRH)-induced gonadotropin secretion and regulates the pubertal activation of GnRH nuerons. A polymorphism in the terminal exon of this mRNA results in two protein isoforms. An adenosine present at the polymorphic site represents the third position in a stop codon. When the adenosine is absent, a downstream stop codon is utilized and the encoded protein extends for an additional seven amino acid residues.
Protein Name:
Metastasis-suppressor KiSS-1
Swissprot Id:
Q15726
Protein Accession #:
NP_002247.3
Nucleotide Accession #:
NM_002256.3
Predicted Species Reactivity:
Human
Sample Type:
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
< 0.078 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Mean Intra-assay CV%: < 3.5% (n = 20)
Mean Inter-assay CV%: < 5.9% (n = 20)
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology KISS1 ELISA Kit (Human) (OKEH01461) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KISS1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KISS1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KISS1 captured in well.
Kit Component:
ComponentAmount
KISS1 Microplate96 Wells (12 x 8 Well strips)
KISS1 Lyophilized Standard2
100X Biotinylated KISS1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Recovery:
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 80%
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express KISS1.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express KISS1.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural human Metastasis-suppressor KiSS-1. No significant cross-reactivity or interference was observed.
Publications:
Platonov, ME, Borovjagin, AV, Kaverina, N., Xiao, T., Kadagidze,Z., Lesniak, M. , Baryshnikova, M. and I. Ulasova. KISS1 tumor suppressor restricts angiogenesis of breast cancer brain metastases and sensitizes them to oncolytic virotherapy in vitro. Cancer Letters. 2018 Mar 28; 417:75-88. ELISA, Human 29269086
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
15 kDa
Protein Size (# AA):
138

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