Catalog No: OKCD00675 (Formerly GWB-KBBQX7)
Size:96WELLS
Price: $650.00
SKU
OKCD00675
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.
Contact Us:
- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for KIR2DS4 ELISA Kit (Human) (OKCD00675) |
---|
Predicted Species Reactivity | Homo sapiens|Human | ||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
ELISA Kit Duration | 3h | ||||||||||||||||||||||
ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
ELISA Kit Range | 1.56-100ng/mL | ||||||||||||||||||||||
ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
ELISA Kit Component |
| ||||||||||||||||||||||
Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
Sample Type | Tissue homogenates, cell lysates, cell culture supernates and other biological fluids. | ||||||||||||||||||||||
Sensitivity | < 0.66 ng/mL | ||||||||||||||||||||||
Specificity | This assay has high sensitivity and excellent specificity for detection of Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). No significant cross-reactivity or interference between Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) and analogues was observed. | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
Gene Symbol | KIR2DS4 |
---|---|
Gene Full Name | killer cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 4 |
Alias Symbols | CD158 antigen-like family member I;CD158I;killer cell immunoglobulin-like receptor 2DS4;killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4;killer inhibitory receptor 4-1-2;KIR antigen 2DS4;KIR1D;KIR-2DS4;KIR412;KKA3;MHC class I NK cell receptor;natural killer-associated transcript 8;NKAT8;NKAT-8;p50 killer cell activating receptor KAR-K1d;P58 natural killer cell receptor clones CL-39/CL-17;p58 NK receptor CL-39/CL-17. |
NCBI Gene Id | 3809 |
Protein Name | Killer cell immunoglobulin-like receptor 2DS4 |
Description of Target | Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. |
Uniprot ID | P43632 |
Protein Accession # | NP_001268900 |
Nucleotide Accession # | NM_001281971.1 |
Protein Size (# AA) | 304 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
Write Your Own Review
We found other products you might like!