Catalog No: OKCD00675 (Formerly GWB-KBBQX7)
Size:96WELLS
Price: $650.00
SKU
OKCD00675
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Datasheets/ManualsPrintable datasheet for KIR2DS4 ELISA Kit (Human) (OKCD00675)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KIR2DS4 Microplate96 Wells (12 x 8 Well Strips)
KIR2DS4 Lyophilized Standard2 x 100 ng
100X Biotinylated KIR2DS4 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sensitivity< 0.66 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4). No significant cross-reactivity or interference between Killer Cell Immunoglobulin Like Receptor 2DS4 (KIR2DS4) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolKIR2DS4
Gene Full Namekiller cell immunoglobulin like receptor, two Ig domains and short cytoplasmic tail 4
Alias SymbolsCD158 antigen-like family member I;CD158I;killer cell immunoglobulin-like receptor 2DS4;killer cell immunoglobulin-like receptor, two domains, short cytoplasmic tail, 4;killer inhibitory receptor 4-1-2;KIR antigen 2DS4;KIR1D;KIR-2DS4;KIR412;KKA3;MHC class I NK cell receptor;natural killer-associated transcript 8;NKAT8;NKAT-8;p50 killer cell activating receptor KAR-K1d;P58 natural killer cell receptor clones CL-39/CL-17;p58 NK receptor CL-39/CL-17.
NCBI Gene Id3809
Protein NameKiller cell immunoglobulin-like receptor 2DS4
Description of TargetReceptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells.
Uniprot IDP43632
Protein Accession #NP_001268900
Nucleotide Accession #NM_001281971.1
Protein Size (# AA)304
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