Catalog No: OKBB00653
Size:96 Wells
Price: $583.00
SKU
OKBB00653
Availability: Domestic: within 1-2 week delivery | International: within 1-2 week delivery
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  • $55: Antibody & Protein in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Please click here to view the MSDS.
COA DatasheetClickHereto download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human Kininogen-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kininogen-1 has been precoated onto 96-well plates. Standards(NS0, Q19-S644) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kininogen-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human Kininogen-1 amount of sample captured in plate.
ELISA Kit Range156 pg/ml - 10,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
Sample123123
n 161616242424
Mean (pg/mL)8.51428101935
SD0.3230.6581.540.430.9692.17
CV%3.84.75.54.35.16.2
ELISA Kit Component
ComponentAmount
96-Well plate Pre-coated with Anti-Human Kininogen-1 Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human Kininogen-1 Standard2 x 10 ng/tube
100X Biotinylated Anti-Human Kininogen-1 Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants, serum and plasma (heparin, EDTA)
Sensitivity<20 pg/ml
SpecificityNatural and recombinant human Kininogen-1
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: NS0; Immunogen sequence: Q19-S644
DescriptionFor quantitative detection of human Kininogen-1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Gene SymbolKNG1
Gene Full Namekininogen 1
Alias SymbolsAlpha-2-thiol proteinase inhibitor, BDK, BK, BK, included, bradykinin, bradykinin, included, Fitzgerald factor, Fitzgerald factor, included, Flaujeac factor, included, High molecular weight kininogen, High molecular weight kininogen, included, HMWK, HMWK, included, Ile-Ser-Bradykinin, Kallidin I, Kallidin II, kininogen 1, KNG, KNG1, KNG1_HUMAN, LMWK, included, Low molecular weight growth-promoting factor, Low molecular weight kininogen, included, Williams factor, included, Williams-Fitzgerald-Flaujeac factor
NCBI Gene Id3827
Protein NameKininogen-1
Description of TargetKininogen-1 (KNG1), also known as alpha-2-thiol proteinase inhibitor, Williams-Fitzgerald-Flaujeac factor or the HMWK-kallikrein factor, is a protein that in humans is encoded by the KNG1 gene. It is mapped to 3q27.3. The KNG1 gene uses alternative splicing to generate two different proteins – high – molecular - weight kininogen (HMWK) and low - molecular- weight kininogen (LMWK). HMWK is essential for blood coagulation and assembly of the kallikrein-kinin system. Also, bradykinin, a peptide causing numerous physiological effects, is released from HMWK. In contrast to HMWK, LMWK is not involved in blood coagulation. In addition to that, KNG1 is a constituent of the blood coagulation system as well as the kinin-kallikrein system.
Uniprot IDP01042
Protein Accession #NP_000884.1
Nucleotide Accession #NM_000893.3
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