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Catalog No: OKCD08801
Size:96WELLS
Price: $800.00
SKU
OKCD08801
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Datasheets/ManualsPrintable datasheet for KIF20A ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 20A (KIF20A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 20A (KIF20A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 20A (KIF20A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kinesin Family, Member 20A (KIF20A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin Family, Member 20A (KIF20A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin Family, Member 20A (KIF20A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
KIF20A Microplate96 Wells (12 x 8 Well strips)
KIF20A Lyophilized Standard2
100X KIF20A HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.049ng/mL
Gene SymbolKIF20A
Gene Full Namekinesin family member 20A
Alias SymbolsMKLP2, RAB6KIFL
NCBI Gene Id10112
Protein NameKinesin-like protein KIF20A
Description of TargetKIF20A interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B.It may act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. KIF20A has a microtubule plus end-directed motility.
Uniprot IDO95235
Protein Accession #NP_005724.1
Nucleotide Accession #NM_005733.2
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