KIF1A ELISA Kit (Human) (OKCD08799)

Catalog No: OKCD08799

Size:96WELLS

KIF1A ELISA Kit (Human) (OKCD08799) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kinesin Family, Member 1A (KIF1A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kinesin Family, Member 1A (KIF1A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kinesin Family, Member 1A (KIF1A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Kinesin Family, Member 1A (KIF1A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Kinesin Family, Member 1A (KIF1A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Kinesin Family, Member 1A (KIF1A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KIF1A Microplate: 96 Wells (12 x 8 Well strips)
  • KIF1A Lyophilized Standard: 2
  • 100X KIF1A HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.051ng/mL

Target Info

• Gene Symbol: KIF1A
• Gene Full Name: kinesin family member 1A
• Alias Symbols: ATSV;axonal transporter of synaptic vesicles;C2orf20;HSN2C;kinesin, heavy chain, member 1A, homolog of mouse;kinesin-like protein KIF1A;microtubule-based motor KIF1A;MRD9;NESCAVS;SPG30;UNC104;unc-104- and KIF1A-related protein.
• NCBI Gene Id: 547
• Protein Name: Kinesin-like protein KIF1A
• Description of Target: Motor for anterograde axonal transport of synaptic vesicle precursors.
• Uniprot ID: Q12756
• Protein Accession #: NP_001230937.1
• Nucleotide Accession #: NM_001244008.1