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Catalog No: OKCD00646 (Formerly GWB-KBBVO7)
Size:96WELLS
Price: $750.00
SKU
OKCD00646
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Datasheets/ManualsPrintable datasheet for KIAA1524 ELISA Kit (Human) (OKCD00646)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit Linearity
Sample1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
serum(n=5)79-98%84-93%92-99%79-99%
EDTA plasma(n=5)79-89%80-98%93-105%95-104%
heparin plasma(n=5)83-102%80-94%80-93%79-98%
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cancerous Inhibitor Of PP2A (CIP2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cancerous Inhibitor Of PP2A (CIP2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Cancerous Inhibitor Of PP2A (CIP2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit Recovery
MatrixRecovery Range (%)Average (%)
serum(n=5)98-105101
EDTA plasma(n=5)92-10196
heparin plasma(n=5)84-9287
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cancerous Inhibitor Of PP2A (CIP2A) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-KIAA1524 Microplate96 Wells (12 x 8 Well Strips)
KIAA1524 Lyophilized Standard2 x 10 ng
100X Biotinylated KIAA1524 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma and other biological fluids
Sensitivity< 0.056 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Cancerous Inhibitor Of PP2A (CIP2A). No significant cross-reactivity or interference between Cancerous Inhibitor Of PP2A (CIP2A) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolCIP2A
Gene Full Namecellular inhibitor of PP2A
Alias Symbolscancerous inhibitor of PP2A;cancerous inhibitor of protein phosphatase 2A;cell proliferation regulating inhibitor of protein phosphatase 2A;KIAA1524;NOCIVA;p90;p90 autoantigen;protein CIP2A.
NCBI Gene Id57650
Protein NameProtein CIP2A
Description of TargetOncoprotein that inhibits PP2A and stabilizes MYC in human malignancies. Promotes anchorage-independent cell growth and tumor formation.
Uniprot IDQ8TCG1
Protein Accession #NP_065941
Nucleotide Accession #NM_020890.2
Protein Size (# AA)905
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