Catalog No: OKEH08527
Size:96T
Price: $725.00
SKU
OKEH08527
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.
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- Toll Free: 888-880-0001
- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit. |
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Predicted Species Reactivity | Homo sapiens, Human | ||||||||||||||||||||||
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Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
ELISA Kit Detection Method | Colorimetric, OD450 nm | ||||||||||||||||||||||
ELISA Kit Principle | Aviva Systems Biology KEAP1 ELISA Kit (Human) (OKEH08527) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KEAP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KEAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KEAP1 captured in well. | ||||||||||||||||||||||
ELISA Kit Range | 78-5000pg/mL | ||||||||||||||||||||||
ELISA Kit Recovery | Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20) | ||||||||||||||||||||||
ELISA Kit Reproducibility | Mean Intra-assay CV%: <=5.5% (n = 20) Mean Inter-assay CV%: <=12.1% (n = 20) | ||||||||||||||||||||||
ELISA Kit Component |
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Additional Information | Function: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome. | ||||||||||||||||||||||
:: | Subcellular Location: Cytoplasm Nucleus Shuttles between cytoplasm and nucleus. | ||||||||||||||||||||||
:: | Unigene: Hs.465870 SMR: Q14145 String: 9606.ENSP00000171111 | ||||||||||||||||||||||
:: | KEGG: hsa:9817 MIM: 606016 Pfam: PF07707 | ||||||||||||||||||||||
Reconstitution and Storage | Store as indicated in product manual. | ||||||||||||||||||||||
Sensitivity | 40.6pg/mL | ||||||||||||||||||||||
Specificity | Natural and recombinant Human Kelch-like ECH-associated protein 1 | ||||||||||||||||||||||
Assay Info | Assay Methodology: Quantitative Sandwich ELISA |
Gene Symbol | KEAP1 |
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Gene Full Name | kelch like ECH associated protein 1 |
Alias Symbols | cytosolic inhibitor of Nrf2;INrf2;KEAP1 delta C;kelch-like ECH-associated protein 1;kelch-like family member 19;kelch-like protein 19;KLHL19. |
NCBI Gene Id | 9817 |
Protein Name | Q14145 |
Description of Target | Kelch-like ECH-associated protein 1 |
Uniprot ID | https://www.uniprot.org/uniprot/Q14145 |
Protein Accession # | NP_036421.2 |
Nucleotide Accession # | NM_012289.3 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
-
See our General FAQ page.
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