Catalog No: OKEH08527
Size:96T
Price: $725.00
SKU
OKEH08527
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KEAP1 ELISA Kit (Human) (OKEH08527)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit PrincipleAviva Systems Biology KEAP1 ELISA Kit (Human) (OKEH08527) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KEAP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KEAP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KEAP1 captured in well.
ELISA Kit Range78-5000pg/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 109% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=5.5% (n = 20)
Mean Inter-assay CV%: <=12.1% (n = 20)
ELISA Kit Component
ComponentAmount
KEAP1 Microplate96 Wells (12 x 8 Well strips)
KEAP1 Lyophilized Standard2
100X Biotinylated KEAP1 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Acts as a substrate adapter protein for the E3 ubiquitin ligase complex formed by CUL3 and RBX1 and targets NFE2L2/NRF2 for ubiquitination and degradation by the proteasome, thus resulting in the suppression of its transcriptional activity and the repression of antioxidant response element-mediated detoxifying enzyme gene expression. Retains NFE2L2/NRF2 and may also retain BPTF in the cytosol. Targets PGAM5 for ubiquitination and degradation by the proteasome.
::Subcellular Location: Cytoplasm Nucleus Shuttles between cytoplasm and nucleus.
::Unigene: Hs.465870
SMR: Q14145
String: 9606.ENSP00000171111
::KEGG: hsa:9817
MIM: 606016
Pfam: PF07707
Reconstitution and StorageStore as indicated in product manual.
Sensitivity40.6pg/mL
SpecificityNatural and recombinant Human Kelch-like ECH-associated protein 1
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKEAP1
Gene Full Namekelch like ECH associated protein 1
Alias Symbolscytosolic inhibitor of Nrf2;INrf2;KEAP1 delta C;kelch-like ECH-associated protein 1;kelch-like family member 19;kelch-like protein 19;KLHL19.
NCBI Gene Id9817
Protein NameQ14145
Description of TargetKelch-like ECH-associated protein 1
Uniprot IDhttps://www.uniprot.org/uniprot/Q14145
Protein Accession #NP_036421.2
Nucleotide Accession #NM_012289.3
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