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Catalog No: OKEH00244
Size:96T
Price: $425.00
SKU
OKEH00244
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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KDR ELISA Kit (Human) : 96 Wells (OKEH00244)

Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology KDR ELISA Kit (Human) (OKEH00244) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KDR has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for KDR is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KDR captured in well.
ELISA Kit Range0.78-50ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.3% (n = 20)
Mean Inter-assay CV%: < 9.7% (n = 20)
ELISA Kit Component
ComponentAmount
KDR Microplate96 Wells (12 x 8 Well strips)
KDR Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated KDR Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.39 ng/mL
SpecificityNatural and recombinant Human Vascular endothelial growth factor receptor 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolKDR
Gene Full Namekinase insert domain receptor
Alias SymbolsCD309;Fetal liver kinase 1;fetal liver kinase-1;FLK1;Kinase insert domain receptor;kinase insert domain receptor (a type III receptor tyrosine kinase);protein-tyrosine kinase receptor Flk-1;soluble VEGFR2;tyrosine kinase growth factor receptor;vascular endothelial growth factor receptor 2;VEGFR;VEGFR2.
NCBI Gene Id3791
Protein NameP35968
Description of TargetVascular endothelial growth factor receptor 2
Uniprot IDhttps://www.uniprot.org/uniprot/P35968
Protein Accession #NP_002244.1
Nucleotide Accession #NM_002253.2
Protein Size (# AA)1356
Molecular Weight152 kDa
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