KDM1A ELISA Kit (Human) (OKEH03137)

Catalog No: OKEH03137

Size:96T

Human KDM1A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology KDM1A ELISA Kit (Human) (OKEH03137) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KDM1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for KDM1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KDM1A captured in well.
• ELISA Kit Range: 0.312-20ng/ml
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.9% (n = 20)
  Mean Inter-assay CV%: <= 8.7% (n = 20)
• ELISA Kit Component:
  • Anti-KDM1A Microplate: 96 Wells (12 x 8 Well strips)
  • KDM1A Lyophilized Standard: 2
  • 100X Biotinylated KDM1A Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
• Sensitivity: 0.179 ng/mL
• Specificity: Natural and recombinant Human Lysine-specific histone demethylase 1A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KDM1A
• Gene Full Name: lysine demethylase 1A
• Alias Symbols: [histone H3]-dimethyl-L-lysine(4) FAD-dependent demethylase 1A;amine oxidase (flavin containing) domain 2;AOF2;BHC110;BRAF35-HDAC complex protein BHC110;CPRF;FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit;flavin-containing amine oxidase domain-containing protein 2;KDM1;LSD1;lysine (K)-specific demethylase 1A;lysine-specific histone demethylase 1;lysine-specific histone demethylase 1A.
• NCBI Gene Id: 23028
• Protein Name: O60341
• Description of Target: Lysine-specific histone demethylase 1A
• Uniprot ID: https://www.uniprot.org/uniprot/O60341
• Protein Accession #: NP_001009999.1 
• Nucleotide Accession #: NM_001009999.2
• Protein Size (# AA): 852
• Molecular Weight: 93 kDa