KDELR1 Antibody : Biotin (OASE00114-Biotin)

Catalog No: OASE00114-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Drosophilia, yeast, Saccharomyce, Plants
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: 2E7
• Isotype: IgG2b
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IF
• Additional Information: Background Info: Detects ~78kDa.
  Scientific Background: HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a
  variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes;
  participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3).
  The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in yeast, plant, nematode and other ER proteins. 2E7 specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of HDEL-containing proteins in 10ug of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: Raised against a synthetic HDEL peptide corresponding to the C-terminus of yeast Bip
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:100-1:1000 (WB), 1:50-1:500 (IF)
• Storage Buffer: PBS pH7.4, 50% glycerol
• Reference: 1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684.
  2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697.
  3. Entrez Gene: HDEL, Gene ID: 10945
  4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

Target Info

• Gene Symbol: KDELR1
• Gene Full Name: ER lumen protein-retaining receptor 1
• Alias Symbols: ERD2, HDEL, PM23, ERD2.1
• NCBI Gene Id: 10945
• Protein Name: ER lumen protein-retaining receptor 1
• Uniprot ID: P24390
• Protein Accession #: NP_006792.1
• Nucleotide Accession #: NM_006801.2