Catalog No: OASE00040 (Formerly GWB-489798)
Size:25ug
Price: $209.00
SKU
OASE00040
Availability: Discontinued
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  • $55: Antibody & Protein in US
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Datasheets/ManualsPrintable datasheet for OASE00040
Product Info
Predicted Species ReactivityHuman, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Pig, Sheep, Dog, Chicken, Drosophila, Xenopus
ClonalityMonoclonal
CloneKR-10
IsotypeIgG1
HostMouse
ConjugationUnconjugated
ApplicationWB, IHC, ICC, IF, IP
Additional InformationBackground Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.
::Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4).
In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
::Certificate of Analysis: 1ug/ml was sufficient for detection of KDEL receptor in 20ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
Reconstitution and StorageStore at -20C. Shipping with Blue Ice or 4C.
ImmunogenA 21 residue synthetic peptide (amino acids 192-212) based on the bovine KDEL receptor and the peptide coupled to KLH
PurificationProtein G Purified
Concentration1 mg/ml
SpecificityDetects ~25kDa.
DilutionWB (1:1000), ICC/IF (1:1000); optimal dilutions for assays should be determined by the user.
Storage BufferPBS pH7.2, 50% glycerol, 0.09% sodium azide
Reference1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737.
2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219.
3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316.
4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328.
5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163.
6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm
DescriptionThe endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4). In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
Gene SymbolKDEL Receptor
Gene Full NameKDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
Alias SymbolsERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23
NCBI Gene Id618184
Uniprot IDP33946
Protein Accession #NP_001069963.1
  1. What is the species homology for "KDEL Receptor Antibody (OASE00040)"?

    The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human, Mouse, Rat, Bovine, Monkey, Hamster, Rabbit, Pig, Sheep, Dog, Chicken, Drosophila, Xenopus".

  2. How long will it take to receive "KDEL Receptor Antibody (OASE00040)"?

    This item is available "Domestic: within 1-2 weeks delivery | International: 1-2 weeks".

  3. What buffer format is "KDEL Receptor Antibody (OASE00040)" provided in?

    This item is provided in "".
    Additional format options may be available. For more information please contact info@avivasysbio.com.

  4. What are other names for "KDEL Receptor Antibody (OASE00040)"?

    This target may also be called "ERD2, ERD2.1, ERD21, HDEL, KDEL, KDEL R1, KDELR1, PM23" in publications.

  5. What is the shipping cost for "KDEL Receptor Antibody (OASE00040)"?

    The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.

  6. What is the guarantee for "KDEL Receptor Antibody (OASE00040)"?

    All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.

  7. Can I get bulk pricing for "KDEL Receptor Antibody (OASE00040)"?

    You can get bulk pricing for this item by going here.

  8. What is the molecular weight of the protein?

    The molecular weight reported by Uniprot for this item is "".
    Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics.

  9. What protocols are available for "KDEL Receptor Antibody (OASE00040)"?

    We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.

  10. What are positive controls for "KDEL Receptor"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  11. What are negative controls for "KDEL Receptor"?

    We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.

  12. What other proteins interact with "KDEL Receptor"?

    This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.

  13. What biological processes are associated with "KDEL Receptor"?

    This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.

  14. What cellular components are associated with "KDEL Receptor"?

    This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.

  15. What protein functions are associated with "KDEL Receptor"?

    This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.

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