KDEL Receptor Antibody : Biotin (OASE00041-Biotin)

Catalog No: OASE00041-Biotin

Size:100ug

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Monkey, Hamster, Rabbit, Pig, Bovine, Sheep, Canine, Chicken, Drosophilia, Xenopus
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer.
• Clonality: Monoclonal
• Clone: KR-10
• Isotype: IgG1
• Host: Mouse
• Conjugation: Biotin
• Application: WB, IP, ICC, IHC, IF
• Additional Information: Background Info: Detects an ~25kDa protein corresponding to the molecular mass of KDEL on SDS-PAGE immunoblots.
  Scientific Background: The endoplasmic reticulum is part of a protein sorting pathway, or in essence, the transportation system of the eukaryotic cell. The majority of endoplasmic reticulum resident proteins are retained in the endoplasmic reticulum through a retention motif. This motif is composed of four amino acids at the C-terminal end of the protein sequence. The most common retention sequence is KDEL (lys-asp-glu-leu). However, variation on KDEL does occur and other sequences can also give rise to endoplasmic reticulum retention (6). There are three KDEL receptors in mammalian cells, all have a very high degree of sequence identity; and all are located within the cis-Golgi and its intermediate compartments (4).
  In terms of function, KDEL receptors interact with GAP (GTPase-activating protein) of ARF1, which is involved in COPI dependent vesicle transport, and the KDEL receptor may also be responsible for the recruitment of this ARF1 to membranes which can then aid in the regulation of vesicle budding (3). It is also important to note that the KDEL receptor exhibits extensive sequence identity o yeast protein Erd2p, which is a receptor for the yeast ER retention signal (4, 5).
  Certificate of Analysis: 1ug/ml was sufficient for detection of KDEL receptor in 20ug monkey Vero cell lysate by colorimetric immunoblot analysis using Goat Anti-Mouse IgG:AP as the secondary.
• Reconstitution and Storage: All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil). Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
• Immunogen: A 21 residue synthetic peptide based on the bovine KDEL receptor and the peptide coupled to KLH
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Dilution: 1:1000 dilution was sufficient for detection by colorimetric analysis
• Storage Buffer: PBS pH7.2, 50% glycerol
• Reference: 1. Whiteman P., and Handford P.A. (2003) Hum Mol Genet 12(7): 727-737.
  2. Forthoffer N., et al. (2002) J Bioenerg Biomemb 34(3): 209-219.
  3. Aoe T., et al. (1997) EMBO J. 16: 7305-7316.
  4. Tang B.L., Wong S.H, Qi X.L. Low S.H., and Hong W. (1993) J. Cell Biol. 120: 325-328.
  5. Lewis M.J. and Pelham H.R. (1990) Nature 348: 162-163.
  6. Spurger L. (2002). Endoplasmic reticulum: Structure and function. University of Texas Medical Branch. Retrieved September 13, 2006, from http://cellbio.utmb.edu/cellbio/rer1.htm

Target Info

• Gene Symbol: Kdelr1
• Gene Full Name: KDEL (Lys-Asp-Glu-Leu) endoplasmic reticulum protein retention receptor 1
• Alias Symbols: Erd2.1, AW215843, 8030486F04Rik
• NCBI Gene Id: 68137
• Uniprot ID: Q99JH8
• Protein Accession #: NP_598711.1