KCNQ1 Antibody (OASE00157)

Catalog No: OASE00157 (Formerly GWB-F10EF8)

Size:100ug

Western Blot analysis of Rat tissue lysate showing detection of KCNQ4 protein using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Mouse Anti-KCNQ4 Monoclonal Antibody, Clone N43/6. Tissue: frozen brain section. Species: mouse. Fixation: 10% Formalin Solution for 12-24 hours at RT. Primary Antibody: Mouse Anti-KCNQ4 Monoclonal Antibody at 1:1000 for 1 hour at RT. Secondary Antibody: HRP/DAB Detection System: Biotinylated Goat Anti-Mouse, Streptavidin Peroxidase, DAB Chromogen (brown) for 30 minutes at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 250-500 ul for 5 minutes at RT.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat, Hamster
• Clonality: Monoclonal
• Clone: N37A/10 (Formerly sold as S37A-10)
• Isotype: IgG1
• Host: Mouse
• Conjugation: Unconjugated
• Application: WB, IHC, ICC, IF, IP, AM
• Additional Information: Background Info: Detects ~75kDa.
  Scientific Background: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). Specifically, Kv7.1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. Kv7.1 is present in the cell membranes of cardiac muscle tissue and in inner ear neurons (3) among other tissues. In the cardiac cells, Kv7.1 mediates the IKs (or slow delayed rectifying K+) current that contributes to the repolarization of the cell, terminating the cardiac action potential and thereby the heart's contraction (4, 5).
  Certificate of Analysis: 1 ug/mL was sufficient for detection of KCNQ1 in 10ug of COS-1 cell lysate transiently expressing KCNQ1 by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
• Reconstitution and Storage: Store at -20C. Shipping with Blue Ice or 4C.
• Immunogen: Fusion protein amino acids 2-101 of human KCNQ1
• Purification: Protein G Purified
• Concentration: 1 mg/ml
• Specificity: Detects ~75kDa.
• Dilution: WB (1:1000), IHC (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Reference: 1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.:Sunderland, MA USA.
  2. www.iochannels.org
  3. Lang F., Vallon V., Knipper M., Wagenmann P. (2007) Am
  J Physiol. 293(4): C1187-1208.
  4. Kurokawa J., et al. (2009) Channels (Austin). 3(1): 16-24.
  5. Silva J., and Rudy Y. (2005) Circulation. 112(10): 1384-
  1391.
• Description: Kv7.1 (KvLQT1) is a potassium channel protein coded for by the gene KCNQ1. Kv7.1 is present in the cell membranes of cardiac muscle tissue and in inner ear neurons (1) among other tissues. In the cardiac cells, Kv7.1 mediates the IKs (or slow delayed rectifying K+) current that contributes to the repolarization of the cell, terminating the cardiac action potential and thereby the heart's contraction (2, 3).

Target Info

• Gene Symbol: KCNQ1
• Gene Full Name: potassium voltage-gated channel, KQT-like subfamily, member 1
• Alias Symbols: ATFB1, ATFB3, FLJ26167, IKs producing slow voltage-gated potassium channel subunit alpha, IKs producing slow voltage-gated potassium channel subunit alpha KvLQT1, Jervell and Lange-Nielsen syndrome 1, JLNS1, KCNA8, KCNA9, KCNQ1, KCNQ1_HUMAN, kidney and cardiac voltage dependend K+ channel, KQT-like 1, Kv1.9, Kv7.1, KVLQT1, long (electrocardiographic) QT syndrome, Ward-Romano syndrome 1, LQT, LQT1, Potassium voltage-gated channel subfamily KQT member 1, potassium voltage-gated channel KQT-like subfamily member 1, RWS, slow delayed rectifier channel subunit, SQT2, Voltage-gated potassium channel subunit Kv7.1, WRS
• NCBI Gene Id: 3784
• Uniprot ID: P51787
• Protein Accession #: NP_000209.2