KCNN2 ELISA Kit (Human) (OKCD09271)

Catalog No: OKCD09271

Size:96WELLS

KCNN2 ELISA Kit (Human) (OKCD09271) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Potassium Intermediate Small Conductance Calcium Activated Channel Subfamily N, Member 2 (KCNN2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • KCNN2 Microplate: 96 Wells (12 x 8 Well strips)
  • KCNN2 Lyophilized Standard: 2
  • 100X KCNN2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.059ng/mL

Target Info

• Gene Symbol: KCNN2
• Gene Full Name: potassium calcium-activated channel subfamily N member 2
• Alias Symbols: apamin-sensitive small-conductance Ca2+-activated potassium channel;hSK2;KCa2.2;potassium channel, calcium activated intermediate/small conductance subfamily N alpha, member 2;potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2;SK2;SKCa 2;SKCA2;small conductance calcium-activated potassium channel 2;small conductance calcium-activated potassium channel protein 2.
• NCBI Gene Id: 3781
• Protein Name: Small conductance calcium-activated potassium channel protein 2
• Description of Target: Forms a voltage-independent potassium channel activated by intracellular calcium. Activation is followed by membrane hyperpolarization. Thought to regulate neuronal excitability by contributing to the slow component of synaptic afterhyperpolarization. The channel is blocked by apamin.
• Uniprot ID: Q9H2S1
• Protein Accession #: NP_001265133.1
• Nucleotide Accession #: NM_001278204.1