KCNJ11 Antibody (OAAF01138)

Catalog No: OAAF01138 (Formerly GWB-ASC535)

Size:100 ug

Western blot analysis of extracts from 3T3 cells, using Kir6.2 Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Kir6.2 Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Kir6.2 Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Kir6.2.
• Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Specificity: Kir6.2 (Ab-224) antibody detects endogenous levels of total Kir6.2 protein.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:3000; IHC: 1:50~1:100; IF: 1:100~1:500; ELISA: 1:10000
• Reference: Inagaki N., Science 270:1166-1170(1995).
  Ribalet B., Biophys. J. 84:266-276(2003).
  Meissner T., Hum. Mutat. 13:351-361(1999).

Target Info

• Gene Symbol: KCNJ11
• Gene Full Name: potassium inwardly rectifying channel subfamily J member 11
• Alias Symbols: ATP-sensitive inward rectifier potassium channel 11;beta-cell inward rectifier subunit;BIR;HHF2;IKATP;inward rectifier K(+) channel Kir6.2;inwardly rectifing potassium channel subfamily J member 11;inwardly rectifying potassium channel KIR6.2;inwardly-rectifying potassium channel subfamily J member 11;KIR6.2;MODY13;PHHI;PNDM2;potassium channel inwardly rectifing subfamily J member 11;potassium channel, inwardly rectifying subfamily J member 11;potassium voltage-gated channel subfamily J member 11;TNDM3.
• NCBI Gene Id: 3767
• Protein Name: ATP-sensitive inward rectifier potassium channel 11
• Description of Target: This receptor is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium (By similarity). Subunit of ATP-sensitive potassium channels (KATP). Can form cardiac and smooth muscle-type KATP channels with ABCC9. KCNJ11 forms the channel pore while ABCC9 is required for activation and regulation.
• Uniprot ID: Q14654
• Protein Accession #: NP_001159762.1
• Molecular Weight: 43 kDa