KCNIP3 Antibody (Phospho-Ser63) (OAAF00212)

Catalog No: OAAF00212 (Formerly GWB-ASB474)

Size:100 ug

Western blot analysis of extracts from K562 cells treated with forskolin 40nM 30' and 293 cells treated with PMA 125ng/ml 30', using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The lane on the right is treated with the synthesized peptide.

Immunohistochemistry analysis of paraffin-embedded human brain, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is treated with the synthesized peptide.

Immunofluorescence analysis of HeLa cells, using Calsenilin/KCNIP3 (Phospho-Ser63) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse
• Product Format: Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Clonality: Polyclonal
• Isotype: IgG
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunocytochemistry|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human Calsenilin/KCNIP3 around the phosphorylation site of Ser63.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: Calsenilin/KCNIP3 (Phospho-Ser63) antibody detects endogenous levels of Calsenilin/KCNIP3 only when phosphorylated at serine 63
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: WB: 1:500~1:1000
  IHC: 1:50~1:100
  IF: 1:100~1:500
  ELISA: 1:20000
• Reference: Terman B.I., Oncogene 6:1677-1683(1991).
  Mitola S., J. Virol. 74:344-353(2000).
  Sjoeblom T., Science 314:268-274(2006)

Target Info

• Gene Symbol: KCNIP3
• Gene Full Name: potassium voltage-gated channel interacting protein 3
• Alias Symbols: A-type potassium channel modulatory protein 3;calsenilin;calsenilin, presenilin-binding protein, EF hand transcription factor;CSEN;DREAM;DRE-antagonist modulator;KCHIP3;Kv channel interacting protein 3, calsenilin;kv channel-interacting protein 3;potassium channel interacting protein 3.
• NCBI Gene Id: 30818
• Protein Name: Calsenilin
• Description of Target: Calcium-dependent transcriptional repressor that binds to the DRE element of genes including PDYN and FOS. Affinity for DNA is reduced upon binding to calcium and enhanced by binding to magnesium. Seems to be involved in nociception (By similarity).
• Uniprot ID: Q9Y2W7
• Protein Accession #: NP_001030086.1
• Molecular Weight: 29 kDa