KATNB1 ELISA Kit (Human) (OKWB00237)

Catalog No: OKWB00237

Size:96T

Human KATNB1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	82-96%	85-100%	89-103%	88-99%
  EDTA Plasma (n=5)	87-99%	82-98%	82-93%	84-97%
  Heparin Plasma (n=5)	82-97%	88-99%	85-99%	83-95%

• ELISA Kit Principle: Aviva Systems Biology KATNB1 ELISA Kit (Human) (OKWB00237) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for KATNB1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for KATNB1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample KATNB1 captured in the well.
• ELISA Kit Range: 0.313-20ng/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	87-93	90
  EDTA Plasma (n=5)	89-103	94
  Heparin Plasma (n=5)	91-103	97

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • KATNB1 Microplate: 96 Wells (12 x 8 Well strips)
  • KATNB1 Lyophilized Standard: 2 x 20 ng
  • 100X KATNB1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 0.188 ng/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: KATNB1
• Gene Full Name: katanin regulatory subunit B1
• Alias Symbols: KAT;katanin (80 kDa);katanin p80 (WD repeat containing) subunit B 1;katanin p80 (WD40-containing) subunit B 1;katanin p80 subunit B1;katanin p80 WD40 repeat-containing subunit B1;katanin p80 WD40-containing subunit B1;LIS6;p80 katanin.
• NCBI Gene Id: 10300
• Protein Name: Katanin p80 WD40 repeat-containing subunit B1
• Description of Target: Participates in a complex which severs microtubules in an ATP-dependent manner. May act to target the enzymatic subunit of this complex to sites of action such as the centrosome. Microtubule severing may promote rapid reorganization of cellular microtubule arrays and the release of microtubules from the centrosome following nucleation. Microtubule release from the mitotic spindle poles may allow depolymerization of the microtubule end proximal to the spindle pole, leading to poleward microtubule flux and poleward motion of chromosome. Microtubule release within the cell body of neurons may be required for their transport into neuronal processes by microtubule-dependent motor proteins. This transport is required for axonal growth.
• Uniprot ID: Q9BVA0
• Protein Accession #: NP_005877
• Nucleotide Accession #: NM_005886