Catalog No: OKEH06006
Size:96 Wells
Price: $525.00
SKU
OKEH06006
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3 Hours
ELISA Kit PrincipleAviva Systems Biology Kat8 ELISA Kit (Rat) (OKEH06006) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Kat8 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and incubated.After washing, a biotinylated detector antibody specific for Kat8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Kat8 captured in well.
ELISA Kit Range0.78 - 50ng/ml
ELISA Kit RecoveryMean recovery when spiking into Serum and Plasma = 104%
ELISA Kit ReproducibilityThree samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
Mean Intra-Assay Precision - <5.1%, n = 20
Mean Inter-Assay Precision - <7.3%, n = 20
ELISA Kit Component
ComponentAmount
KAT8 Microplate96 Wells (12 x 8 Well strips)
KAT8 Lyophilized Standard2
100X Biotinylated KAT8 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma and other biological fluids
Sensitivity0.412 ng/mL
SpecificityNatural and recombinant Rat Histone acetyltransferase KAT8
Assay InfoAssay Methodology: Quantitative Competitive ELISA
Gene SymbolKAT8
Gene Full NameLysine acetyltransferase 8
Alias SymbolsMyst1
NCBI Gene Id310194
Protein NameHistone acetyltransferase KAT8
Description of TargetHistone acetyltransferase which may be involved in transcriptional activation. May influence the function of ATM. As part of the MSL complex it is involved in acetylation of nucleosomal histone H4 producing specifically H4K16ac. As part of the NSL complex it may be involved in acetylation of nucleosomal histone H4 on several lysine residues. That activity is less specific than the one of the MSL complex. Can also acetylate TP53/p53 at 'Lys-120'.
Uniprot IDQ5XI06
Protein Accession #NP_001017378.1
Nucleotide Accession #NM_001017378.1
Protein Size (# AA)458
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