KAP ELISA Kit (Mouse) (OKEH01949)

Catalog No: OKEH01949

Size:96T

Mouse KAP ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Competitive
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology KAP ELISA Kit (Mouse) (OKEH01949) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-mouse KAP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KAP and incubated. The KAP found in the sample or standards competes with the biotinylated KAP for limited binding sites on the immobilized anti-mouse KAP antibody. Excess unbound biotinylated KAP and sample or standard KAP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KAP captured in the well and inversely proportional to the amount of KAP which was contained in the sample or standard.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.8% (n = 20)
  Mean Inter-assay CV%: < 7.9% (n = 20)
• ELISA Kit Component:
  • Anti-KAP Microplate: 96 Wells (12 x 8 Well strips)
  • KAP Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X KAP-Biotin Complex: 1 x 60 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Biotin Complex Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.078 ng/mL
• Specificity: Natural and recombinant Mouse Kidney androgen-regulated protein
• Assay Info: Assay Methodology: Quantitative Competitive ELISA

Target Info

• Gene Symbol: Kap
• Gene Full Name: kidney androgen regulated protein
• Alias Symbols: ARP;AW146435;FKSG22;kidney androgen-regulated protein.
• NCBI Gene Id: 16483
• Protein Name: P61110
• Description of Target: Kidney androgen-regulated protein
• Uniprot ID: https://www.uniprot.org/uniprot/P61110
• Protein Accession #: NP_034724.1
• Nucleotide Accession #: NM_010594.2
• Protein Size (# AA): 121
• Molecular Weight: 13 kDa