JUP ELISA Kit (Human) (OKCD08040)

Catalog No: OKCD08040

Size:96WELLS

JUP ELISA Kit (Human) (OKCD08040) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Junction Plakoglobin (JUP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Junction Plakoglobin (JUP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Junction Plakoglobin (JUP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Junction Plakoglobin (JUP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Junction Plakoglobin (JUP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • JUP Microplate: 96 Wells (12 x 8 Well strips)
  • JUP Lyophilized Standard: 2
  • 100X JUP HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056ng/mL

Target Info

• Gene Symbol: JUP
• Gene Full Name: junction plakoglobin
• Alias Symbols: catenin (cadherin-associated protein), gamma 80kDa;Catenin gamma;CTNNG;desmoplakin III;desmoplakin-3;desmosomal protein 3;DP3;DPIII;junction plakoglobin;PDGB;PG;PKGB.
• NCBI Gene Id: 3728
• Protein Name: Junction plakoglobin
• Description of Target: Common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity).
• Uniprot ID: P14923
• Protein Accession #: NP_002221.1
• Nucleotide Accession #: NM_002230.2