JMJD6 Antibody (OAPB00955)

Catalog No: OAPB00955

Size:100UG

Western blot analysis of JMJD6 in human brain tissue lysate with JMJD6 antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of JMJD6 in rat brain tissue with JMJD6 antibody at 2.5 ug/mL.

Product Info

• Predicted Species Reactivity: Bovine|Chicken|Human|Mouse|Rat
• Product Format: Liquid. JMJD6 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: JMJD6 antibody can be stored at 4C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: JMJD6 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human JMJD6. The immunogen is located within amino acids 70 - 120 of JMJD6.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-JMJD6 antibody is Catalog # OEPB03071
• Application Info: JMJD6 antibody can be used for detection of JMJD6 by Western blot at 1 - 2 ug/ml. (Optimal dilution should be determined by user)
• Reference: Takeuchi T, Watanabe Y, Takano-Shimizu T, et al. Roles of jumonji and jumonji family genes in chromatin regulation and development. Dev. Dyn.2006; 235:2449-59.;Fadok VA, Bratton DL, Rose DM, et al. A receptor for phosphatidylserine-specific clearance of apoptotic cells. Nature2000; 405:85-90.;Zakharova L, Dadsetan S, and Fomina AF. Endogenous JMJD6 gene product is expressed at the cell surface and regulates phagocytosis in immature monocyte-like activated THP-1 cells. J. Cell. Phys.2009; 221:84-91.;Bose J, Gruber AD, Helming L, et al. The phosphatidylserine receptor has essential functions during embryogenesis but not in apoptotic cell removal. J. Biol.2004; 3:15.

Target Info

• Gene Symbol: JMJD6
• Gene Full Name: jumonji domain containing 6, arginine demethylase and lysine hydroxylase
• Alias Symbols: arginine demethylase and lysine hydroxylase;bifunctional arginine demethylase and lysyl-hydroxylase JMJD6;histone arginine demethylase JMJD6;jmjC domain-containing protein 6;jumonji domain-containing protein 6;lysyl-hydroxylase JMJD6;peptide-lysine 5-dioxygenase JMJD6;phosphatidylserine receptor;PSR;PTDSR;PTDSR1.
• NCBI Gene Id: 23210
• Protein Name: Bifunctional arginine demethylase and lysyl-hydroxylase JMJD6
• Description of Target: Dioxygenase that can both act as a histone arginine demethylase and a lysyl-hydroxylase. Acts as a lysyl-hydroxylase that catalyzes 5-hydroxylation on specific lysine residues of target proteins such as U2AF2/U2AF65 and LUC7L2. Acts as a regulator of RNA splicing by mediating 5-hydroxylation of U2AF2/U2AF65, affecting the pre-mRNA splicing activity of U2AF2/U2AF65. In addition to peptidyl-lysine 5-dioxygenase activity, may act as an RNA hydroxylase, as suggested by its ability to bind single strand RNA. Also acts as an arginine demethylase which demethylates histone H3 at 'Arg-2' (H3R2me) and histone H4 at 'Arg-3' (H4R3me), thereby playing a role in histone code. However, histone arginine demethylation may not constitute the primary activity in vivo. Has no histone lysine demethylase activity. Required for differentiation of multiple organs during embryogenesis. Acts as a key regulator of hematopoietic differentiation: required for angiogenic sprouting by regulating the pre-mRNA splicing activity of U2AF2/U2AF65. Seems to be necessary for the regulation of macrophage cytokine responses.
• Uniprot ID: Q6NYC1