JMJD3 Antibody - N-terminal region (OAAB07251)

Catalog No: OAAB07251

Size:200ul

Western blot analysis of anti-JMJD3 (N-term) Pab (OAAB07251) in Hela cell line lysates. JMJD3 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human brain tissue reacted with JMJD3 (N-term) (Cat.#OAAB07251), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Confocal immunofluorescent analysis of JMJD3 Antibody (N-term) (Cat#OAAB07251) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, IF
• Additional Information: Precautions: JMJD3 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Histone demethylase that specifically demethylates 'Lys- 27' of histone H3, thereby playing a central role in histone code Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation
  Cellular Location: Nucleus (Probable).
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This JMJD3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human JMJD3.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50
  IF: 1:10~50

Target Info

• Gene Symbol: KDM6B
• Alias Symbols: JMJD3, NEDCFSA
• NCBI Gene Id: 23135
• Protein Name: Lysine-specific demethylase 6B
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: O15054
• Protein Accession #: NP_001073893.1
• Molecular Weight: 176632 Da