JMJD2D Antibody - C-terminal region (ARP35946_T100)

Catalog#: ARP35946_T100

• Size: 100 ul

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Human
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Clonality: Polyclonal
• Host: Rabbit
• Application: WB
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Gene Symbol: KDM4D
• Official Gene Full Name: Lysine (K)-specific demethylase 4D
• NCBI Gene Id: 55693
• Protein Name: Lysine-specific demethylase 4D
• Swissprot Id: Q6B0I6
• Protein Accession #: NP_060509
• Nucleotide Accession #: NM_018039
• Alias Symbols: JMJD2D
• Description of Target: JMJD2D belongs to JMJD2 family that is consist of four cancer-associated genes
• Protein Size (# AA): 523
• Molecular Weight: 59kDa
• Purification: Protein A purified
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express JMJD2D.
• Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human JMJD2D
• Predicted Homology Based on Immunogen Sequence: Human: 100%
• Peptide Sequence: Synthetic peptide located within the following region: SSTPGPSAQIIHPSNGRRGRGRPPQKLRAQELTLQTPAKRPLLAGTTCTA
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: HECW2; PPP1CC; CUL4B; TP53; AR; KDM4D;
• Blocking Peptide: For anti-KDM4D (ARP35946_T100) antibody is Catalog # AAP35946 (Previous Catalog # AAPP07204)
• Target Reference: Katoh,M. et al., (2004) Int. J. Oncol. 24 (6), 1623-1628
• Publications:

  Kim, T.-D. et al. Pro-growth role of the JMJD2C histone demethylase in HCT-116 colon cancer cells and identification of curcuminoids as JMJD2 inhibitors. Am. J. Transl. Res. 6, 236-47 (2014). WB, Human 24936217

  Kim, T.-D., Oh, S., Shin, S. & Janknecht, R. Regulation of tumor suppressor p53 and HCT116 cell physiology by histone demethylase JMJD2D/KDM4D. PLoS One 7, e34618 (2012). WB, Human 22514644

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

• IHC Tips & Tricks
• ICC Tips & Tricks
• ELISA Tips & Tricks
• WB/IB Tips & Tricks
• IP Tips & Tricks

See our General FAQ page.

Product Protocols: JMJD2D antibody tested with Human Hepg2 Cells (ARP35946_T100)