JMJD2A Antibody (OAPB00951)

Catalog No: OAPB00951

Size:100UG

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Western blot analysis of JMJD2A in rat liver tissue lysate with JMJD2A antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of JMJD2A in rat liver tissue with JMJD2A antibody at 5 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. JMJD2A Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunohistochemistry-Paraffin|Western blot
  Positive Controls: Rat Liver Tissue Lysate
• Reconstitution and Storage: JMJD2A antibody can be stored at 4C for three months and -20C, stable for one year. As with all antibodies, care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: JMJD2A antibody was raised against a 14 amino acid synthetic peptide from near the center of human JMJD2A. The immunogen is located within amino acids 340 - 390 of JMJD2A.
• Purification: Affinity Purified
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-JMJD2A antibody is Catalog # OEPB03069
• Application Info: WB 1-2 ug/mL
  IHC-P 5 ug/mL
• Reference: Gray SG, Iglesias AH, Lizcano F, et al. Functional characterization of JMJD2A, a histone deacetylase- and retinoblastoma-binding protein. J. Biol. Chem.2005; 31:28507-18.;
  Shin S and Janknecht R. Activation of androgen receptor by histone demethylases JMJD2A and JMJD2D. Biochem. Biophys. Res. Comm.2007; 359:742-6.;

Target Info

• Gene Symbol: KDM4A
• Gene Full Name: lysine demethylase 4A
• Alias Symbols: [histone H3]-trimethyl-L-lysine(36) demethylase 4A;[histone H3]-trimethyl-L-lysine(9) demethylase 4A;JHDM3A;jmjC domain-containing histone demethylation protein 3A;JMJD2;JMJD2A;jumonji C domain-containing histone demethylase 3A;jumonji domain containing 2;jumonji domain containing 2A;jumonji domain-containing protein 2A;lysine (K)-specific demethylase 4A;lysine-specific demethylase 4A;TDRD14A;tudor domain containing 14A.
• NCBI Gene Id: 9682
• Protein Name: jumonji domain containing 2A [Homo sapiens]|Lysine-specific demethylase 4A
• Description of Target: Histone demethylase that specifically demethylates 'Lys-9' and 'Lys-36' residues of histone H3, thereby playing a central role in histone code (PubMed:26741168). Does not demethylate histone H3 'Lys-4', H3 'Lys-27' nor H4 'Lys-20'. Demethylates trimethylated H3 'Lys-9' and H3 'Lys-36' residue, while it has no activity on mono- and dimethylated residues. Demethylation of Lys residue generates formaldehyde and succinate. Participates in transcriptional repression of ASCL2 and E2F-responsive promoters via the recruitment of histone deacetylases and NCOR1, respectively.
• Uniprot ID: O75164
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/CAH71021