Catalog No: OKCD04513
Size:96WELLS
Price: $825.00
SKU
OKCD04513
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Datasheets/ManualsPrintable datasheet for JKAMP ELISA Kit (Rat) (OKCD04513)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to JNK1/MAPK8 Associated Membrane Protein (JKAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain JNK1/MAPK8 Associated Membrane Protein (JKAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of JNK1/MAPK8 Associated Membrane Protein (JKAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range1.56-100ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level JNK1/MAPK8 Associated Membrane Protein (JKAMP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
EPG Microplate96 Wells (12 x 8 Well strips)
EPG Lyophilized Standard2
100X EPG HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
100X Luminol Substrate1 x 2 mL
Substrate Diluent1 x 20 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity0.61 ng/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolJkamp
Gene Full NameJNK1/MAPK8-associated membrane protein
Alias SymbolsJNK1/MAPK8-associated membrane protein;RGD1308917.
NCBI Gene Id299127
Protein NameJNK1/MAPK8-associated membrane protein
Description of TargetJNK1/MAPK8-associated membrane protein
Uniprot IDD3ZZT8
Protein Accession #NP_001100208.1
Nucleotide Accession #NM_001106738.1
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