JHDM2a Antibody - C-terminal region (OAAB08608)

Catalog No: OAAB08608

Size:400ul

JHDM2a Antibody (C-term) (Cat. #OAAB08608) western blot analysis in UM cell line lysates (35ug/lane).This demonstrates the JHDM2a antibody detected the JHDM2a protein (arrow)

JHDM2a Antibody (C-term) (Cat. #OAAB08608)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of JHDM2a Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Product Info

• Predicted Species Reactivity: Human, Mouse, Rat
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC
• Additional Information: Precautions: JHDM2a Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Histone demethylase that specifically demethylates 'Lys- 9' of histone H3, thereby playing a central role in histone code Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1
  Cellular Location: Cytoplasm (By similarity). Nucleus (By similarity). Note=Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity)
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This JHDM2a antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 976-1008 amino acids from the C-terminal region of human JHDM2a.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse; Rat
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:10~50

Target Info

• Gene Symbol: KDM3A
• Alias Symbols: TSGA, JMJD1, JHDM2A, JHMD2A, JMJD1A
• NCBI Gene Id: 55818
• Protein Name: Lysine-specific demethylase 3A
• Description of Target: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q9Y4C1
• Protein Accession #: NP_001140160.1
• Molecular Weight: 147341 Da