Catalog No: OPPA01477 (Formerly GWB-721816)
Size:100UG
Price: $192.00
SKU
OPPA01477
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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- Phone: 858-552-6979
- Email: info@avivasysbio.com
Shipping Info:
- $55: Antibody & Protein in US
- $55 + $25/Kit in US
- Contact us for international orders.
Datasheets/Manuals | Printable datasheet for OPPA01477 |
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Predicted Species Reactivity | Japanese Encephalitis Virus |
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Product Format | Liquid 20 mM Tris-MES (pH 6.5), 8 M urea, 200 mM NaCl & 0.05% Tween-20 |
Application | ELISA, WB |
Reconstitution and Storage | JEV, although stable at 4°C for one week, should be stored below -18°C. Please prevent freeze/thaw cycles. |
Purification | Purified by proprietary chromatographic techniques. |
Concentration | 1 mg/ml |
Purity | Encephalitis protein is >90% pure as determined by SDS-PAGE (coomassie staining) |
Specificity | Immunoreactive with sera of encephalitis virus infected individuals. |
Application Info | Encephalitis antigen is suitable for ELISA and Western blots, excellent antigen for detection of JEV with minimal specificity problems. |
Tag | The protein is fused to a 6-histidine tag |
Gene Symbol | Encephalitis Japanese |
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Alias Symbols | JEV, Japanese Encephalitis Virus ENV Recombinant |
Description of Target | Japanese encephalitis previously known as Japanese B encephalitis is a virus from the family Flaviviridae. it is closely related to the West Nile virus and St. Louis encephalitis virus. Positive sense single stranded RNA genome is packaged in the capsid, formed by the capsid protein. The outer envelope is formed by envelope (E) protein and is the protective antigen. It aids in entry of the virus to the inside of the cell. The genome also encodes several nonstructural proteins also (NS1,NS2a,NS2b,NS3,N4a,NS4b,NS5). NS1 is produced as secretory form also. NS3 is a putative helicase, and NS5 is the viral polymerase. It has been noted that the Japanese encephalitis virus (JEV) infects the lumen of the endoplasmic reticulum (ER) and rapidly accumulates substantial amounts of viral proteins for the JEV. Japanese Encephalitis is diagnosed by detection of antibodies in serum and CSF (cerebrospinal fluid) by IgM capture ELISA. The E.coli derived recombinant protein contains full length Japanese Encephalitis virus ENV antigen. |
Protein Size (# AA) | Recombinant |
Molecular Weight | 50 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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