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JARID2 Antibody (OAEB00423)

100 ug
$229.00
In Stock
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Gene Symbol:
JARID2
NCBI Gene Id:
3720
Protein Name:
Protein Jumonji
Swissprot Id:
Q92833
Protein Accession #:
NP_004964.2
Alias Symbols:
JARID2, jumonji, AT rich interactive domain 2, JMJ, OTTHUMP00000016058, jumonji homolog, jumonji, AT rich interactive domain 2 protein, jumonji-like protein
Replacement Item:
This antibody may replace item sc-47604 from Santa Cruz Biotechnology.
Host:
Goat
Clonality:
Polyclonal
Application:
ELISA
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express JARID2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express JARID2.
Predicted Species Reactivity:
Cow, Guinea Pig, Human, Mouse, Rabbit, Zebrafish
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Guinea Pig: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Zebrafish: 78%
Complete computational species homology data:
JARID2 Antibody (OAEB00423)
Peptide Sequence:
C-HRSAQDLRKQVSKVN
Product Format:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Reconstitution and Storage:
Aliquot and store at -20C. Minimize freezing and thawing.
Datasheets/Manuals:
Printable datasheet for anti-JARID2 antibody - OAEB00423
Purity:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Application Info:
Peptide ELISA: antibody detection limit dilution 1:32000.
Western Blot: Preliminary experiments gave an approx 29kDa band in Mouse and Rat Spinal Cord and in Mouse Fetal Brain lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 137kDa according to Mouse NP_068678.1. The 29kDa band was successfully blocked by incubation with the immunizing peptide.
Target Reference:
* Bovill E, Westaby S, Reji S, Sayeed R, Crisp A, Shaw T.; Induction by left ventricular overload and left ventricular failure of the human Jumonji gene (JARID2) encoding a protein that regulates transcription and reexpression of a protective fetal program.; The Journal of thoracic and cardiovascular surgery 2008 Sep 136 (3): 709-16.; Pubmed 18805276

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