Catalog No: OKBB00616
Size:96 Wells
Price: $400.00
Availability: Domestic: within 1 week delivery | International: 1 week
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva's human JAM-A ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for JAM-A has been precoated onto 96-well plates. Standards(E.coli, S28-V238) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for JAM-A is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The absorbance of yellow is proportional to the human JAM-A amount of sample captured in plate.
ELISA Kit Range46.9 pg/ml - 3,000 pg/ml
ELISA Kit Reproducibility
Intra-Assay PrecisionInter-Assay Precision
n 161616242424
Mean (pg/mL)5721145196363113982120
ELISA Kit Component
96-Well plate Pre-coated with Anti-Human JAM-A Antibody1 x (12 x 8 Well Strip)
Lyophilized Recombinant Human JAM-A Standard2 x 10 ng/tube
100X Biotinylated Anti-Human JAM-A Antibody1 x 130 uL
100X Avidin-Biotin-Peroxidase Complex (ABC)1 x 130 uL
Sample Diluent Buffer1 x 30 mL
Antibody Diluent Buffer1 x 12 mL
ABC Diluent Buffer1 x 12 mL
TMB Color Developing Agent1 x 10 mL
TMB Stop Solution1 x 10 mL
10X Wash Buffer1 x 30 mL
Reconstitution and StorageStore at 4C for 6 months, at -20C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Sample TypeCell culture supernatants and serum
Sensitivity<10 pg/ml
SpecificityNatural and recombinant human JAM-A
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
ImmunogenExpression system for standard: E.coli; Immunogen sequence: S28-V238
DescriptionFor quantitative detection of human JAM-A in cell culture supernatants and serum.
Gene SymbolF11R
Gene Full NameF11 receptor
Alias SymbolsCD 321, CD321, CD321 antigen, ESTM33, F11 receptor, F11R, JAM 1, JAM A, JAM, JAM-1, JAM-A, JAM1, JAM1_HUMAN, JAMA, JCAM, Jcam1, Junction adhesion molecule 1, Junction adhesion molecule, mouse, homolog of, Junctional adhesion molecule 1, Junctional adhesion molecule A, KAT, Ly106, PAM 1, PAM-1, PAM1, Platelet adhesion molecule 1, Platelet adhesion molecule, Platelet F11 receptor, PRO301, UNQ264, UNQ264/PRO301
NCBI Gene Id50848
Protein NameJunctional adhesion molecule A
Description of TargetJunctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
Swissprot IdQ9Y624
Protein Accession #NP_058642.1
Nucleotide Accession #NM_016946.4
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express F11R.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express F11R.
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