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JAKMIP2 ELISA Kit (Human) (OKWB00236)

96 Wells
$400.00
In Stock
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Predicted Species Reactivity:
Human
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
JAKMIP2
Alias Symbols:
CTCL tumor antigen HD-CL-04, JAMIP2, Janus kinase and microtubule-interacting protein 2, KIAA0555, NECC1, Neuroendocrine long coiled-coil protein 1
NCBI Gene Id:
9832
Protein Name:
Janus kinase and microtubule-interacting protein 2
Swissprot Id:
Q96AA8
Protein Accession #:
NP_001257863
Nucleotide Accession #:
NM_001270934
Sample Type:
Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
Sensitivity:
0.094 ng/mL
Kit Range:
0.156 - 10 ng/mL
Kit Reproducibility:
Mean Intra-Assay Precision <10%
Mean Inter-Assay Precision <12%
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology JAKMIP2 ELISA Kit (Human) (OKWB00236) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for JAKMIP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for JAKMIP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample JAKMIP2 captured in the well.
Kit Component:
ComponentAmount
JAKMIP2 Microplate96 Wells (12 x 8 Well strips)
JAKMIP2 Lyophilized Standard2 x 10 ng
100X JAKMIP2 Biotinylated Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 20 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Linearity:
Sample Type1:2 Dilution1:4 Dilution1:8 Dilution1:16 Dilution
Serum (n=5)91-105%85-100%89-101%91-102%
EDTA Plasma (n=5)88-100%84-99%90-100%84-99%
Heparin Plasma (n=5)80-91%83-96%82-97%87-98%
Kit Recovery:
Sample TypeRangeMean
Serum (n=5)87-10495
EDTA Plasma (n=5)85-10393
Heparin Plasma (n=5)85-10497
Kit Detection Method:
Colorimetric, OD 450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express JAKMIP2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express JAKMIP2.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Assay Info:
Assay Type: Quantitative Sandwich ELISA

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