JAK1 ELISA Kit (Rat) (OKCD04482)

Catalog No: OKCD04482

Size:96WELLS

Rat JAK1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Janus Kinase 1 (JAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Janus Kinase 1 (JAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Janus Kinase 1 (JAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Janus Kinase 1 (JAK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.625-40ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average (%)
  Serum (n=5)	82-93	88
  EDTA Plasma (n=5)	89-99	94
  Heparin Plasma (n=5)	89-98	94

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Janus Kinase 1 (JAK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Janus Kinase 1 (JAK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • LOX Microplate: 96 Wells (12 x 8 Well strips)
  • LOX Lyophilized Standard: 2
  • 100X LOX HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: 0.238 ng/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Jak1
• Gene Full Name: Janus kinase 1
• Alias Symbols: Janus protein tyrosine kinase 1;tyrosine-protein kinase JAK1.
• NCBI Gene Id: 84598
• Protein Name: Tyrosine-protein kinase
• Description of Target: protein-tyrosine kinases; involved in intracellular signal transduction [RGD, Feb 2006]
• Uniprot ID: G3V9W2
• Protein Accession #: NP_445918.1
• Nucleotide Accession #: NM_053466.1