JAG2 ELISA Kit (Mouse) (OKEH08526)

Catalog No: OKEH08526

Size:96T

Mouse JAG2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	107-115% (n = 20)	95-105% (n = 20)	109-120% (n = 20)	110-120% (n = 20)
  EDTA plasma(n=5)	97-107% (n = 20)	96-107% (n = 20)	93-102% (n = 20)	84-95% (n = 20)
  heparin plasma(n=5)	96-105% (n = 20)	103-113% (n = 20)	89-97% (n = 20)	108-118% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology JAG2 ELISA Kit (Mouse) (OKEH08526) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for JAG2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for JAG2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample JAG2 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 99% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=7.5% (n = 20)
  Mean Inter-assay CV%: <=8.9% (n = 20)
• ELISA Kit Component:
  • JAG2 Microplate: 96 Wells (12 x 8 Well strips)
  • JAG2 Lyophilized Standard: 2
  • 100X Biotinylated JAG2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Putative Notch ligand involved in the mediation of Notch signaling. Plays an essential role during limb, craniofacial and thymic development. May be involved in myogenesis and in the development of peripheral and central nervous systems.
  Subcellular Location: Membrane Single-pass type I membrane protein
  Unigene: Mm.186146
  SMR: Q9QYE5
  String: 10090.ENSMUSP00000075224
  KEGG: mmu:16450
  Pfam: PF01414
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.088ng/mL
• Specificity: Natural and recombinant Mouse Protein jagged-2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Jag2
• Gene Full Name: jagged 2
• Alias Symbols: D12Ggc2e;mJagged2-1;protein jagged-2;Se;Serh;sm;syndactylism.
• NCBI Gene Id: 16450
• Protein Name: Q9QYE5
• Description of Target: Protein jagged-2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9QYE5
• Protein Accession #: NP_034718.2
• Nucleotide Accession #: NM_010588.2