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ITGB3 Antibody (OASA02310)

  • Catalog#: OASA02310
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  • This product is Genway GWB-F55CE1
Predicted Species Reactivity:
Human
Product Format:
Purified IgG - liquid
Clonality:
Monoclonal
Clone:
PM6/13
Isotype:
IgG1
Host:
Mouse
Application:
IHC-AFF, FC, IP, WB
Additional Information:
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma cell line.
Histology Positive Control Tissue: Bone marrow
:::
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
:::
Approx Protein Conc: IgG concentration 1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
Reconstitution and Storage:
Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Gene Symbol:
ITGB3
NCBI Gene Id:
3690
Protein Name:
Integrin beta-3
Swissprot Id:
P05106
Protein Accession #:
NP_000203.2
Alias Symbols:
CD61, GP3A, GPIIIa, ITGB3
Replacement Item:
This antibody may replace item sc-13579 from Santa Cruz Biotechnology.
Description of Target:
MOUSE ANTI HUMAN CD61
Protein Size (# AA):
788
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express ITGB3.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express ITGB3.
Immunogen:
The immunogen for anti-ITGB3 antibody: platelet plasma membrane
Predicted Homology Based on Immunogen Sequence:
Human
Datasheets/Manuals:
Printable datasheet for anti-ITGB3 antibody - OASA02310
Specificity:
CD61
Application Info:
Flow Cytometry:    1/100 - 1/200
Western Blotting(1):    Non-reducing conditions
(1)Special conditions apply. - See [*].
Application Data:
Application #1: Staining of human peripheral blood platelets with Mouse anti Human CD61:Alexa Fluor 647
Application #2: Staining of human peripheral blood platelets with Mouse anti Human CD61:FITC
Application #3: Staining of human peripheral blood platelets with Mouse anti Human CD61:RPE
Protocol Information:
Citation: 1: McHugh BJ, Buttery R, Lad Y, Banks S, Haslett C, Sethi T. Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum.J Cell Sci. 2010 Jan 1;123(Pt 1):51-61. PubMed PMID: 20016066; PubMed Central PMCID: PMC2794710.
Species: Human
Experiment Name: Antibody
Experiment Background: Integrins respond to cytoplasmic signals through changes in integrin-ligand affinity, termed `inside-out' signalling. This experiment highlights a novel mechanism for integrin activation by Fam38A, utilising calpain and R-Ras signalling from the ER. These data represent the first description of a novel spatial regulator of R-Ras, of an alternative integrin activation-suppression pathway based on direct relocalisation of R-Ras to the ER, and of a mechanism linking R-Ras and calpain signalling from the ER with modulation of integrin-ligand affinity.
Experimental Steps: Fam38A rabbit polyclonal antibodies were generated from the peptide sequence RAPNGPEANPVKQLC (Genosphere). 2.TS2/16 β1-integrin activating antibody was purified from a hybridoma clone (ATCC). 3.PAC-1 (αIIbβ3-activation specific) , Anti-Tac-α5 antibody (ACT-1 RPE) and mouse anti-CD29 (K20) conjugated to fluorescein isothiocyanate (FITC) ; Anti-mouse IgM-FITC; Mouse anti-CD29 (HUTS-21) conjugated to phycoerythrin (PE) ; Mouse anti-calpain 1 and rabbit anti-human Calpain S1; Mouse anti-talin (8D4) was collected from different sources.4. Mouse anti-CD61 (β3 integrin) was collected from.5. All other antibodies are listed in supplementary material Table S1.
Number Of Protocols: 1
Target Reference:
1. Michelson, A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons. Leucocyte Typing V. Oxford University Press p 1230-1231.
2. Phillips, D.R. et al. (1991) GPIIb-IIIa: the responsive integrin. Cell 65: 359-362.
3. Hynes, R.O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69: 11-25.

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