- Predicted Species Reactivity:
- Product Format:
- Purified IgG - liquid
- IHC-AFF, FC, IP, WB
- Additional Information:
- Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells from the SP2/0 mouse myeloma cell line.
Histology Positive Control Tissue: Bone marrow
- Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Preservative Stabilisers: 0.09% - Sodium Azide
- Approx Protein Conc: IgG concentration 1 mg/ml
Buffer Solutions: Phosphate buffered saline pH7.4
- Reconstitution and Storage:
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- NCBI Gene Id:
- Protein Name:
- Integrin beta-3
- Alias Symbols:
- CD61, GP3A, GPIIIa, ITGB3
- Replacement Item:
- This antibody may replace item sc-13579 from Santa Cruz Biotechnology.
- Description of Target:
- MOUSE ANTI HUMAN CD61
- Protein Size (# AA):
- Tissue Tool:
- Find tissues and cell lines supported by DNA array analysis to express ITGB3.
- RNA Seq:
- Find tissues and cell lines supported by RNA-seq analysis to express ITGB3.
- The immunogen for anti-ITGB3 antibody: platelet plasma membrane
- Predicted Homology Based on Immunogen Sequence:
- Printable datasheet for anti-ITGB3 antibody - OASA02310
- Application Info:
- Flow Cytometry: 1/100 - 1/200
Western Blotting(1): Non-reducing conditions
(1)Special conditions apply. - See [*].
- Application Data:
- Application #1: Staining of human peripheral blood platelets with Mouse anti Human CD61:Alexa Fluor 647
Application #2: Staining of human peripheral blood platelets with Mouse anti Human CD61:FITC
Application #3: Staining of human peripheral blood platelets with Mouse anti Human CD61:RPE
- Protocol Information:
- Citation: 1: McHugh BJ, Buttery R, Lad Y, Banks S, Haslett C, Sethi T. Integrin activation by Fam38A uses a novel mechanism of R-Ras targeting to the endoplasmic reticulum.J Cell Sci. 2010 Jan 1;123(Pt 1):51-61. PubMed PMID: 20016066; PubMed Central PMCID: PMC2794710.
Experiment Name: Antibody
Experiment Background: Integrins respond to cytoplasmic signals through changes in integrin-ligand affinity, termed `inside-out' signalling. This experiment highlights a novel mechanism for integrin activation by Fam38A, utilising calpain and R-Ras signalling from the ER. These data represent the first description of a novel spatial regulator of R-Ras, of an alternative integrin activation-suppression pathway based on direct relocalisation of R-Ras to the ER, and of a mechanism linking R-Ras and calpain signalling from the ER with modulation of integrin-ligand affinity.
Experimental Steps: Fam38A rabbit polyclonal antibodies were generated from the peptide sequence RAPNGPEANPVKQLC (Genosphere). 2.TS2/16 β1-integrin activating antibody was purified from a hybridoma clone (ATCC). 3.PAC-1 (αIIbβ3-activation specific) , Anti-Tac-α5 antibody (ACT-1 RPE) and mouse anti-CD29 (K20) conjugated to fluorescein isothiocyanate (FITC) ; Anti-mouse IgM-FITC; Mouse anti-CD29 (HUTS-21) conjugated to phycoerythrin (PE) ; Mouse anti-calpain 1 and rabbit anti-human Calpain S1; Mouse anti-talin (8D4) was collected from different sources.4. Mouse anti-CD61 (β3 integrin) was collected from.5. All other antibodies are listed in supplementary material Table S1.
Number Of Protocols: 1
- Target Reference:
- 1. Michelson, A. D. et al. (1995) A panel of platelet mAb for the study of haemostasis and thrombosis in baboons. Leucocyte Typing V. Oxford University Press p 1230-1231.
2. Phillips, D.R. et al. (1991) GPIIb-IIIa: the responsive integrin. Cell 65: 359-362.
3. Hynes, R.O. (1992) Integrins: versatility, modulation, and signaling in cell adhesion. Cell 69: 11-25.