ITGB1 Antibody (OASA01804)

Catalog No: OASA01804 (Formerly GWB-FF82F5)

Size:25UG

Staining of human peripheral blood monocytes with Mouse anti Human CD29

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. Phosphate buffered saline
• Clonality: Monoclonal
• Clone: 12G10
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunofluorescence|Immunohistochemistry-Frozen
• Additional Information: Fusion Partners: Spleen cells from an immunised BALB/c mice were fused with cells of the X63/Ag8.653 mouse myeloma cell line.
  Approx Protein Conc: IgG concentration 1.0 mg/ml
  Buffer Solutions: Phosphate buffered saline
  Preservative Stabilisers: 0.09% - Sodium Azide
• Reconstitution and Storage: -20°C
• Immunogen: Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract
• Purification: Protein G Purified
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: 1 mg/ml
• Specificity: Mouse anti Human CD29 monoclonal antibody, clone 12G10 recognizes human CD29 also known as beta1 integrin or VLA-4 subunit alpha. CD29 is a ~130kDa under reducing, ~115kda under non-reducing conditions single pass type I transmembrane glycoprotein. CD29 acts as the common beta subunit of the heterodimeric very late antigens 1-6, complexing with CD49a-f respectively where it forms part of the receptors for laminin , collagen and fibronectin. the VLA heterodimers ediate cell-cell and cell-matrix interactions.
  Mouse anti human CD29, clone 12G10 binding to cells adhering via VLA-4 results in actin cytoskeletal disruption and subsequent inhibition of attachment and spreading whilst 12G10 binding to cells adhering via VLA-5 results in enhancement of both these processes (Humphries et al. 2005). Clone 12G10 enhances alpha 5 beta 1-fibronectin interactions and binds to a region of CD25 contining the binding epitopes of several other anti CD29 antibody clones. However, unlike these, binding of 12G10 is enhanced in the presence of ligands such as fibronectin fragments (Mould et al. 1995). Binding of antibody clone 12G10 to the integrin β1 subunit is affected by divalent cations and the binding epitiope appears to be located around residues 207-218 in the b1 subunit putative A-domain (Mould et al. 1998)
• Application Info: Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
  Suggested Dilutions:
  Flow Cytometry: 1/25 - 1/50
  ELISA: 10ug/ml
• Application Data: Application #1: Staining of human peripheral blood monocytes with Mouse anti Human CD29
• Reference: 1. Sodek, K.L. et al. (2009) Compact spheroid formation by ovarian cancer cells is associated with contractile behavior and an invasive phenotype. Int J Cancer. 124: 2060-70.
  2. Mould, A.P. et al. (1995) Regulation of integrin alpha5 beta1 function by anti-integrin antibodies and divalent cations. Biochem. Soc. Trans. 23: 395S.
  3. Mould, A.P. et al. (1995) Identification of a novel anti-integrin monoclonal antibody that recognises a ligand-induced binding site epitope on the Beta 1 subunit. FEBS Letters 363: 118-122.
  

Target Info

• Gene Symbol: ITGB1
• Gene Full Name: integrin, beta 1
• Alias Symbols: CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA
• NCBI Gene Id: 3688
• Protein Name: Integrin beta-1
• Description of Target: MOUSE ANTI HUMAN CD29
  
• Uniprot ID: P05556
• Protein Accession #: NP_002202.2
• Protein Size (# AA): 798