ITGAX Antibody (OATA00599)

Catalog No: OATA00599

Human peripheral blood monocytes were stained with 5 uL (0.5 ug) violetFluor 450 Anti-Human CD11c (OATA00599) (solid line) or 0.5 ug violetFluor 450 Mouse IgG1 isotype control (dashed line).

Product Info

• Tested Species Reactivity: Human
• Predicted Species Reactivity: Baboon, Chimpanzee, Cynomolgus, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: 3.9
• Isotype: Mouse IgG1, kappa
• Host: Mouse
• Application: FC
  Excitation Laser: Violet (405 nm)
  Emmision: 450 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD11c
• Application Info: This antibody preparation has been pre-titrated and quality-tested for flow cytometry using an appropriate cell type. The antibody has been diluted for use at 5 uL per test, defined as the amount of antibody that will stain a cell sample in a final volume of approximately 100 uL. The number of cells within a sample should be determined empirically, but typically ranges between 1x10e5 to 1x10e8 cells.violetFluor 450 dye is excited by the violet (405 nm) laser and has a peak emission of 450 nm. The most common band pass filters for this dye are 440/40 or 450/50. violetFluor 450 can be used as an alternative for Pacific Blue, BD Horizon V450 or eFluor 450.
• Reference:

  Robinson BA, Estep RD, Messaoudi I, Rogers KS, and Wong SW. J. Virol. 2012. 86:2197-2211. (Flow cytometry - rhesus macaque)

  Campillo-Gimenez L, Laforge M, Fay M, Brussel A, et. al. 2010. J. Virol. 84(4):1838-1846. (Flow cytometry - African green monkey, rhesus macaque)

  Sadhu C, Hendrickson L, Dick KO, Potter TG, and Staunton DE. 2008. J. Immunoassay Immunochem. 29(1):42-57. (in vitro blocking)

  Arndt S, Melle C, Mondal K, Klein G, von Eggeling F, and Bosserhoff A-K. 2007. J. Leukoc. Biol. 82:1466-1472. (Immunoprecipitation)

  McGreal EP, Ikewaki N, Akatsu H, Morgan BP, and Gasque P. 2002. 168:5222-5232. (Immunofluorescence microscopy - frozen tissue)

Target Info

• Gene Symbol: ITGAX
• Gene Full Name: integrin subunit alpha X
• Alias Symbols: CD11C, SLEB6
• NCBI Gene Id: 3687
• Protein Name: integrin alpha-X
• Description of Target: This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene.
• Uniprot ID: P20702
• Protein Accession #: NP_000878.2
• Nucleotide Accession #: NM_000887.4