Catalog No: OKEH04178
Size:96T
Price: $525.00
SKU
OKEH04178
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ITGAV ELISA Kit (Human) (OKEH04178) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ITGAV has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ITGAV is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ITGAV captured in the well.
ELISA Kit Range0.312-20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 6.3% (n = 20)
Mean Inter-assay CV%: <= 8.9% (n = 20)
ELISA Kit Component
ComponentAmount
ITGAV Microplate96 Wells (12 x 8 Well Strips)
ITGAV Standard2 x 2 ng
Sample Diluent1 x 20 mL
ITGAV Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
Sensitivity0.11 ng/mL
SpecificityNatural and recombinant Human Integrin alpha-V
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolITGAV
Gene Full Nameintegrin subunit alpha V
Alias Symbolsantigen identified by monoclonal antibody L230;CD51;integrin alpha-V;integrin alphaVbeta3;integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51);MSK8;Vitronectin receptor;vitronectin receptor subunit alpha;VNRA;VTNR.
NCBI Gene Id3685
Protein NameP06756
Description of TargetIntegrin alpha-V
Uniprot IDhttps://www.uniprot.org/uniprot/P06756
Protein Accession #NP_001138471.1
Nucleotide Accession #NM_001144999.2
Protein Size (# AA)1048
Molecular Weight116 kDa
Write Your Own Review
You're reviewing:ITGAV ELISA Kit (Human) (OKEH04178)
Your Rating
We found other products you might like!