ITGAM/ITGAM Antibody : PerCP-Cy5.5 (OATA00473)

Catalog No: OATA00473

C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Hu/Mo CD11b (OATA00473) (solid line) or 0.25 ug PerCP-Cy5.5 Rat IgG2b isotype control (dashed line).

C57Bl/6 bone marrow cells were stained with 0.25 ug PerCP-Cy5.5 Anti-Mouse CD11b (M1/70) manufactured by Aviva Systems Biology (left panel) or eBioscience (right panel).

Product Info

• Tested Species Reactivity: Human, Mouse
• Predicted Species Reactivity: Chimpanzee, Baboon, Cynomolgus, Rhesus
• Product Format: Liquid. 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2
• Clonality: Monoclonal
• Clone: M1/70
• Isotype: Rat IgG2b, kappa
• Host: Rat
• Conjugation: PERCP: Peridinin Chlorophyll Protein Complex, CY5.5: Cyanine 5.5
• Application: FC
  Excitation Laser: Blue (488 nm)
  Emmision: 676 nm
• Reconstitution and Storage: 2-8C protected from light
• Specificity: CD11b
• Application Info: This antibody preparation has been quality-tested for flow cytometry using mouse spleen cells, or an appropriate cell type (where indicated). Please refer to the figure legend for the optimal concentration used to stain the tissue shown. We recommend titrating the antibody under your specific conditions to determine the optimal concentration of antibody needed in your experimental system.
• Reference:

  Lefort CT, Rossaint J, Moser M, Petrich BG, Zarbock A, Monkley SJ, Critchley DR, Ginsberg MH, Fassler R, and Ley K. 2012. Blood. 119:4275-4282. (in vitro blocking)

  Grewal JS, Pilgrim MJ, Grewal S, Kasman L, Werner P, Bruorton ME, London SD, and London L. 2011. FASEB J. 25:1680-1696. (Immunofluorescence microscopy - frozen tissue)

  Kim W-K, Sun Y, Do H, Autissier P, Halpern EF, Piatak M, Lifson JD, Burdo TH, McGrath MS, and Williams K. 2010. J. Leukoc. Biol. 87: 557-567. (Flow Cytometry - Rhesus macaque)

  Roland CL, Dineen SP, Lynn KD, Sullivan LA, Dellinger MT, Sadegh L, Sullivan JP, Shames DS, and Brekken RA. 2009. Mol. Cancer Ther. 8:1761-1771. (Immunofluorescence microscopy - frozen tissue)

  Sorg H, Lorch B, Jaster R, Fitzner B, Ibrahim S, Holzhueter S, Nizze H, and Vollmar B. 2008. Am. J. Physio. Gastrointest. Liver Physiol. 295: G1274-1280. (Immunohistochemistry - formalin-fixed paraffin embedded tissue)

  Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, and Song W-C. 2008. Blood. 112:1109-1119. (in vivo blocking)

  Ou R, Zhang M, Huang L, Flavell RA, Koni PA, and Moskophidis D. 2008. J. Virol. 82:2952-2965. (Immunohistochemistry - OCT embedded frozen tissue)

  Nutt SL, Metcalf D, D"™Amico A, Polli M, and Wu L. 2005. J. Exp. Med. 201:221-231. (Immunomagnetic bead depletion)

  Whiteland JL, Nicholls SM, Shimeld C, Easty DL, Williams NA, and Hill TJ. 1995. J. Histochem. Cytochem. 43:313-320. (Immunohistochemistry - frozen tissue, paraffin embedded tissue)

  Miller LJ, Schwarting R, and Springer TA. 1986. J. Immunol. 137:2891-2900. (Immunoprecipitation)

Target Info

• Gene Symbol: ITGAM, ITGAM
• Gene Full Name: integrin alpha M
• Alias Symbols: CR, CR3, CR3A, Ly-4, MAC1, Mac-, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik
• NCBI Gene Id: 16409; 3684
• Protein Name: integrin alpha-M