ITGAM Antibody (OAEE00615)

Catalog No: OAEE00615

Size:100T

Immunoprecipitation of human CD11b/CD18 heterodimer from the lysate of washed PBMC isolated from healthy donor.
Lysate was subjected to affinity column chromatography using anti-human CD11b (MEM-174) immunosorbent. Eluted immunoprecipitate was resolved on 7.5% SDS-PAGE and stained with Coomasie Blue.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: MEM-174
• Isotype: IgG2a
• Host: Mouse
• Conjugation: R-PE
• Application: Flow cytometry
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Human granulocytes
• Purification: Purified by size-exclusion chromatography
• Concentration: 20 ul / test
• Specificity: The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type I transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The CD11b mediates neutrophil and monocyte interactions with stimulated endothelium.
  HLDA VI; WS Code BP 310
  HLDA VI; WS Code M 18
• Formulation: PE
• Storage Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
• Reference: Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int Immunol. 2007 May;19(5):675-84.
  Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).
  Lawrence PK, Srikumaran S.: CD11b of Ovis canadensis and Ovis aries: molecular cloning and characterization.Vet Immunol Immunopathol. 2007 Oct 15;119(3-4):287-98.
  Hentzen ER, Neelamegham S, Kansas GS, Benanti JA, McIntire LV, Smith CW, Simon SI. Sequential binding of CD11a/CD18 and CD11b/CD18 defines neutrophil capture and stable adhesion to intercellular adhesion molecule-1. Blood. 2000 Feb 1;95(3):911-20.
  Akramiene D, Kondrotas A, Didziapetriene J, Kevelaitis E.: Effects of beta-glucans on the immune system. Medicina (Kaunas). 2007;43(8):597-606.

Target Info

• Gene Symbol: ITGAM
• Gene Full Name: integrin subunit alpha M
• Alias Symbols: antigen CD11b (p170);CD11 antigen-like family member B;CD11B;cell surface glycoprotein MAC-1 subunit alpha;complement component 3 receptor 3 subunit;CR-3 alpha chain;CR3A;integrin alpha-M;integrin, alpha M (complement component 3 receptor 3 subunit);leukocyte adhesion receptor MO1;MAC-1;MAC1A;macrophage antigen alpha polypeptide;macrophage-1 antigen alpha subunit;MO1A;Neutrophil adherence receptor;neutrophil adherence receptor alpha-M subunit;SLEB6.
• NCBI Gene Id: 3684
• Protein Name: Integrin alpha-M
• Description of Target: Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration (PubMed:28807980). In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (PubMed:21193407). May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity).
• Uniprot ID: P11215
• Protein Accession #: NP_000623.2; NP_001139280.1
• Nucleotide Accession #: NM_000632.3; NM_001145808.1